Postnatal Development of Mouse Liver: Increasing RNA Polymerase Activity and Orotic Acid Incorporation

RNA polymerase activity and incorporation of 3H-orotic acid into RNA in livers of young mice were investigated. Total RNA polymerase activity of isolated liver nuclei increased approximately three-fold during the first 30 days after birth. Assays with α-amanitin revealed that both RNA polymerase I a...

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Bibliographic Details
Published inDifferentiation (London) Vol. 2; no. 5; pp. 269 - 273
Main Authors Bagshaw, J.C., Bond, Bernice H.
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier B.V 01.10.1974
Blackwell Publishing Ltd
Subjects
Age Factors
Amanitins
Animals
Animals, Newborn
Cell Nucleus - metabolism
Chromatography
DNA-Directed RNA Polymerases - analysis
DNA-Directed RNA Polymerases - metabolism
Liver - enzymology
Liver - growth & development
Liver - metabolism
Male
Mice
Orotic Acid - metabolism
RNA - biosynthesis
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Summary:RNA polymerase activity and incorporation of 3H-orotic acid into RNA in livers of young mice were investigated. Total RNA polymerase activity of isolated liver nuclei increased approximately three-fold during the first 30 days after birth. Assays with α-amanitin revealed that both RNA polymerase I and II activity increased over this period. Chromatographic fractionation of solubilized RNA polymerases confirmed that the ratio of polymerase I to polymerase II activity was similar at 1 day and at 30 days of age. Incorporation of 3H-orotic acid into total liver RNA in intact animals also increased during this period. The results are compared with the increase in cell number and cell size in postnatal liver development.
ISSN:0301-4681
1432-0436
DOI:10.1111/j.1432-0436.1974.tb00361.x