Identification of gene products of the P1 operon of Mycoplasma pneumoniae

Gene P1 of Mycoplasma pneumoniae, which codes for a major adhesin, is flanked by two sequences with open reading frames designated ORF4 and ORF6 (Inamine et al., 1988b). In order to identify proteins translated from those ORFs, gene fusions between the N-terminus of the RNA replicase of the Escheric...

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Bibliographic Details
Published inMolecular microbiology Vol. 5; no. 2; p. 299
Main Authors Sperker, B, Hu, P, Herrmann, R
Format Journal Article
LanguageEnglish
Published England 01.02.1991
Subjects
Adhesins, Bacterial
Animals
Antibodies, Bacterial - biosynthesis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Blotting, Western
Cloning, Molecular
Cross Reactions
Escherichia coli - genetics
Genes, Bacterial
Mice
Mycoplasma pneumoniae - genetics
Open Reading Frames
Operon
Protein Biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
RNA Replicase - genetics
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Summary:Gene P1 of Mycoplasma pneumoniae, which codes for a major adhesin, is flanked by two sequences with open reading frames designated ORF4 and ORF6 (Inamine et al., 1988b). In order to identify proteins translated from those ORFs, gene fusions between the N-terminus of the RNA replicase of the Escherichia coli bacteriophage MS2 and selected regions of ORF4 and ORF6 were constructed. The corresponding fusion proteins synthesized in Escherichia coli were used to immunize mice. Antisera directed against ORF4-related sequences did not recognize M. pneumoniae antigens in Western blot analysis, but antisera directed against ORF-6-derived fusion proteins reacted with two M. pneumoniae proteins of 40 kDa and 90 kDa. In addition, some of the antisera also recognized proteins that formed in a sodium dodecyl sulphate/polyacrylamide gel a protein ladder between 115 and 145 kDa.
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1991.tb02110.x