Open fronted safety cabinets in ventilated laboratories

• Published in: Journal of applied bacteriology Vol. 69; no. 3; p. 338
• Main Authors: Clark, R P, Osborne, R W, Pressey, D C, Grover, F, Keddie, J R, Thomas, C
• Format: Journal Article
• Language: English
• Published: England 01.09.1990
• Subjects: Bacteriological Techniques - instrumentation; Containment of Biohazards - instrumentation; Humans; Laboratories - standards; United Kingdom; Ventilation - methods
• ISSN: 0021-8847
• DOI: 10.1111/j.1365-2672.1990.tb01525.x

Open fronted Class I and II microbiological safety cabinets (MSCs) are required by the British Standard 5726 to provide similar levels of operator protection (viz. 10(5). In laboratories that are naturally ventilated large numbers of both types of cabinets have been shown to exceed this requirement consistently over a number of years. The designs of some mechanically ventilated laboratories, however, produce excessive turbulence and draughts that can prejudice containment at the front aperture. On-site commissioning tests to determine operator protection factor are now well established and are recognized as being essential to the setting up of all open fronted cabinets in both ventilated and unventilated laboratories. This paper shows that where environmental conditions induce unsatisfactory cabinet containment, adjustments to air supply and exhaust systems can be made which will enable both Class I and II cabinets to produce operator protection factors in excess of 10(5). When compatibility is achieved between the local environment and the cabinets it is demonstrated that disturbances at the front aperture, caused by operator working procedures or by disturbances due to personnel movement within the room, have similar effects on both Class I and II cabinets. Once performance levels have been satisfactorily achieved, regular containment testing has shown that consistent performance can be maintained. These aspects of open fronted safety cabinet performance are discussed in relation to ventilated laboratories suitable for work with the human immunodeficiency virus (HIV). Of paramount importance in the future is the necessity to design laboratory air systems that will be compatible with satisfactory safety cabinet performance--a relatively new requirement in ventilation system specifications.