Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (α-Al2O3) and titanium (Ti-6Al-4V) alloy substrates

• Published in: Journal of biomedical materials research. Part A Vol. 94A; no. 3; pp. 913 - 926
• Main Authors: Kapoor, Renu, Sistla, Pavana Goury, Kumar, Jerald Mahesh, Raj, T. Avinash, Srinivas, G., Chakraborty, Jui, Sinha, Mithlesh K., Basu, Debabrata, Pande, Gopal
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2010; Wiley-Blackwell
• Subjects: Aluminum Oxide - chemistry; Animals; biocompatibility; Biological and medical sciences; biomimetic coatings; Biotechnology; Body Fluids - chemistry; bone formation; Cattle; Cell Adhesion; Ceramics - chemistry; Coated Materials, Biocompatible - chemistry; Durapatite - chemistry; Female; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Industrial applications and implications. Economical aspects; Male; Materials Testing; Medical sciences; Miscellaneous; Rats; Rats, Wistar; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; tissue engineering; Titanium - chemistry; X-Ray Diffraction; Biological properties; Titanium alloy; Rat; Tissue engineering; Rodentia; Hydroxyapatite; biomimetic coatings; Biological activity; Oxide ceramics; Coated material; Vertebrata; Mammalia; Animal; Biocompatibility; Biomaterial; bone formation; Alumina; Comparative study; Osteogenesis; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32767

Previous reports have shown the use of hydroxyapatite (HAp) and related calcium phosphate coatings on metal and nonmetal substrates for preparing tissue‐engineering scaffolds, especially for osteogenic differentiation. These studies have revealed that the structural properties of coated substrates are dependent significantly on the method and conditions used for coating and also whether the substrates had been modified prior to the coating. In this article, we have done a comparative evaluation of the structural features of the HAp coatings, prepared by using simulated body fluid (SBF) at 25°C for various time periods, on a nonporous metal substrate titanium–aluminium–vanadium (Ti‐6Al‐4V) alloy and a bioinert ceramic substrate alpha‐alumina (α‐Al2O3), with and without their prior treatment with the globular protein bovine serum albumin (BSA). Our analysis of these substrates by scanning electron microscopy (SEM), X‐ray diffraction (XRD), and Fourier‐transform infrared (FTIR) spectrometry showed significant and consistent differences in the quantitative and qualitative properties of the coatings. Interestingly, the bioactivity of these substrates in terms of supporting in vitro cell adhesion and spreading, and in vivo effects of implanted substrates, showed a predictable pattern, thus indicating that some coated substrates prepared under our conditions could be more suitable for biological/biomedical applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010