Isolation and purification procedures for the preparation of paralytic shellfish poisoning toxin standards

Paralytic shellfish poisoning (PSP) is caused by mixtures of saxitoxin analogs of which more than eighteen are known. Reliable and sensitive analytical methods and assays for these toxins are essential to protect the consumer and the shellfish industry, but research has been restricted by a shortage...

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Bibliographic Details
Published inNatural toxins Vol. 2; no. 4; p. 175
Main Authors Laycock, M.V. (Institute for Marine Biosciences, Halifax, Nova Scotia, Canada.), Thibault, P, Ayer, S.W, Walter, J.A
Format Journal Article
LanguageEnglish
Published United States 1994
Subjects
Animals
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
Chromatography, High Pressure Liquid
CROMATOGRAFIA LIQUIDA ALTA PRESION
CYANOPHYTA
Dinoflagellida
DINOPHYCEAE
ELECTROFORESIS
ELECTROPHORESE
Electrophoresis, Paper
ENFERMEDADES TRANSM POR ALIMENTOS
ENVENENAMIENTO
ESPECTROMETRIA
ESPECTROMETRIA DE MASAS
ESPECTROSCOPIA RMN
INTOXICATION
Magnetic Resonance Spectroscopy
MALADIE TRANSMISSIBLE PAR ALIMENT
Marine Toxins - isolation & purification
Marine Toxins - toxicity
PEIGNE
PURIFICACION
PURIFICATION
Reference Standards
Saxitoxin - analogs & derivatives
Saxitoxin - isolation & purification
Saxitoxin - toxicity
Shellfish
SPECTROMETRIE
SPECTROMETRIE DE MASSE
SPECTROSCOPIE RMN
TOXINAS
TOXINE
VIEIRA
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Summary:Paralytic shellfish poisoning (PSP) is caused by mixtures of saxitoxin analogs of which more than eighteen are known. Reliable and sensitive analytical methods and assays for these toxins are essential to protect the consumer and the shellfish industry, but research has been restricted by a shortage of the pure compounds. Only saxitoxin has so far been generally available as a PSP toxin standard, yet sulfated analogs usually occur in higher concentrations than saxitoxin in toxic marine algae and shellfish. Methods are described for the purification of some of the common PSP toxins, in quantities sufficient for the preparation of PSP standards from the dinoflagellate Alexandrium excavatum, the giant sea scallop (Placopecten megallanicus) hepatopancreas, and the cyanobacterium Aphanizomenon flos-aquae. Purity was monitored by high performance liquid chromatography with fluorescence detection (HPLC-FD), ion-spray mass spectrometry (ISP-MS), capillary electrophoresis (CE), and proton NMR spectroscopy
Bibliography:Q03
9563475
ISSN:1056-9014
1522-7189
DOI:10.1002/nt.2620020405