Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization

Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a...

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Bibliographic Details
Published inMolecular ecology notes Vol. 8; no. 1; pp. 164 - 167
Main Authors HARDESTY, B.D, HUGHES, S.L, RODRIGUEZ, V.M, HAWKINS, J.A
Format Journal Article
LanguageEnglish
Published Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2008
Blackwell Publishing Ltd
Subjects
Cactaceae
conservation
Echinocactus
microsatellite repeats
microsatellites
natural resource management
polymorphism
SSR
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Summary:Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.
Bibliography:http://dx.doi.org/10.1111/j.1471-8286.2007.01913.x
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content type line 23
ISSN:1471-8278
1755-098X
1471-8286
1755-0998
DOI:10.1111/j.1471-8286.2007.01913.x