Inhibition of monoamine oxidase by 7-chloro-4-nitrobenzofurazan

7-Chloro-4-nitrobenzofurazan (NBD-Cl) is a potent inhibitor of both types of monoamine oxidase (MAO). NBD-Cl competitively inhibited the oxidative deamination of kynuramine catalyzed by human placenta MAO-A, the oxidative deamination of benzylamine catalyzed by bovine liver MAO-B, the oxidative deam...

Full description

Saved in:
Bibliographic Details
Published inJournal of neurochemistry Vol. 55; no. 3; p. 813
Main Authors Fu, R C, Liu, X F, Chen, S U
Format Journal Article
LanguageEnglish
Published England 01.09.1990
Subjects
Animals
Benzofurans - pharmacology
Binding, Competitive
Brain - enzymology
Cattle
Female
Hydrogen-Ion Concentration
Kinetics
Mitochondria - enzymology
Mitochondria, Liver - enzymology
Monoamine Oxidase Inhibitors - pharmacology
Placenta - enzymology
Placenta - ultrastructure
Pregnancy
Rats
Online AccessGet more information

Cover

More Information
Summary:7-Chloro-4-nitrobenzofurazan (NBD-Cl) is a potent inhibitor of both types of monoamine oxidase (MAO). NBD-Cl competitively inhibited the oxidative deamination of kynuramine catalyzed by human placenta MAO-A, the oxidative deamination of benzylamine catalyzed by bovine liver MAO-B, the oxidative deamination of serotonin catalyzed by rat brain MAO-A, and the oxidative deamination of phenylethylamine catalyzed by rat brain MAO-B. In addition, a time-dependent inactivation of MAOs by NBD-Cl has been demonstrated upon incubation of the enzyme preparations with NBD-Cl at pH 9, but not at pH 7.5. The time-dependent inhibition of MAO by NBD-Cl could be prevented by the addition of 4-nitrophenyl azide, an active site-directed label of MAO, during incubation of the enzyme with NBD-Cl. On the basis of these findings, it is suggested that at pH 9, NBD-Cl modifies one (or more) essential lysine residue(s) in the active sites of the two types of MAO.
ISSN:0022-3042
DOI:10.1111/j.1471-4159.1990.tb04564.x