Isolation and characterization of microsatellite loci in Psilopeganum sinense Hemsl (Rutaceae), an endangered herb endemic to Yangtze River valley
Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from...
Saved in:
| Published in | Molecular ecology notes Vol. 8; no. 1; pp. 227 - 229 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
2008
Blackwell Publishing Ltd |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from two to eight, with an average value of 3.7. The ranges of observed and expected heterozygosities were 0.000-0.750 and 0.365-0.800, respectively. These microsatellite loci have been directly applied to the ongoing conservation genetic studies of P. sinense. |
|---|---|
| AbstractList | Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from two to eight, with an average value of 3.7. The ranges of observed and expected heterozygosities were 0.000-0.750 and 0.365-0.800, respectively. These microsatellite loci have been directly applied to the ongoing conservation genetic studies of P. sinense. Abstract Twenty‐nine primer pairs flanking microsatellite repeats were designed from an AC‐enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from two to eight, with an average value of 3.7. The ranges of observed and expected heterozygosities were 0.000–0.750 and 0.365–0.800, respectively. These microsatellite loci have been directly applied to the ongoing conservation genetic studies of P. sinense . |
| Author | YE, QIGANG HUANG, HONGWEN TANG, FEIYAN YAO, XIAOHONG |
| Author_xml | – sequence: 1 fullname: TANG, FEIYAN – sequence: 2 fullname: YE, QIGANG – sequence: 3 fullname: YAO, XIAOHONG – sequence: 4 fullname: HUANG, HONGWEN |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21585763$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNUU1v1DAUjFAR_YC_AL4BEgl2HMfOgQOqyraoLWhpheBiOc7L1ktiL3ZStv0Z_GIcUvaML37Pb2asN3OY7FlnIUkQwRmJ5-06IwUnqchFmeUY8wyTitJs-yg52A32djUX-8lhCGuMCa_y4kmynxMmGC_pQfL7LLhODcZZpGyD9I3ySg_gzf386FrUG-1dUAN0nRkAdU4bZCz6HEznNrBSduxRMBZsAHQKfejQq-U4KA0KXr-Jqghso-wKPDToBnw99RBF0eDQtzgY7gEtzS14dKu6Du6eJo9b1QV49nAfJdcfTq6OT9PzT4uz4_fnqS4opmlBdNXoQvC2LCnXGHQjaM5riHuJPKcFZaQmTDEsWtAtrxirmpbUmFJOW5bTo-TlrLvx7ucIYZC9CTpuqSy4MUhRcp4TwVlEihk5GRE8tHLjTa_8nSRYToHItZy8lpPvcgpE_g1EbiP1-cMnY91DsyP-SyAC3s2AXybu_t_C8uLkcqoiP535Jgyw3fGV_yFLTjmTXy8Xkn6_uCJi8VEuI_7FjG-Vk2rlTZDXX3JMKMaCUlZh-geRv7VM |
| CitedBy_id | crossref_primary_10_1093_aob_mct255 crossref_primary_10_1111_jse_12208 crossref_primary_10_1016_j_bse_2014_03_034 |
| Cites_doi | 10.1093/oxfordjournals.jhered.a111573 10.1046/j.1365-294x.1998.00374.x 10.1046/j.0962-1083.2001.01418.x 10.1007/s10592-005-9073-x |
| ContentType | Journal Article |
| Copyright | 2007 The Authors 2007 The Authors. |
| Copyright_xml | – notice: 2007 The Authors – notice: 2007 The Authors. |
| DBID | FBQ BSCLL NPM AAYXX CITATION 7X8 |
| DOI | 10.1111/j.1471-8286.2007.01933.x |
| DatabaseName | AGRIS Istex PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic CrossRef PubMed |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Ecology |
| EISSN | 1471-8286 1755-0998 |
| EndPage | 229 |
| ExternalDocumentID | 10_1111_j_1471_8286_2007_01933_x 21585763 MEN1933 ark_67375_WNG_3ZMT18GJ_R US201300833590 |
| Genre | article Journal Article |
| GroupedDBID | .GA .Y3 10A 123 1OC 29M 31~ 36B 4.4 51W 51X 52N 52O 52P 52S 52T 52W 52X 53G 7PT 8-1 8-4 8-5 930 A03 AAEVG AAHHS AANLZ AAXRX AAZKR ABCUV ABJNI ABPTK ABTAH ACAHQ ACBWZ ACCFJ ACCZN ACGFS ACNCT ACPOU ACXBN ACXME ACXQS ADAWD ADDAD ADEOM ADIZJ ADMGS ADOZA AEEZP AEIGN AEIMD AEQDE AETEA AEUQT AEUYR AFBPY AFEBI AFFNX AFFPM AFGKR AFPWT AFVGU AFZJQ AGJLS AIURR AIWBW AJBDE ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AVWKF AZFZN BDRZF BRXPI BY8 CAG CO8 COF CS3 D-F DCZOG DRFUL DRSTM EAS EBS ECGQY EJD ESX F00 F01 F04 F5P FBQ FEDTE G-S GODZA HF~ HVGLF HZI H~9 IHE LATKE LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MRFUL MRSTM MSFUL MSSTM MVM MXFUL MXSTM P4D QB0 ROL SUPJJ TN5 UB1 WIH WIK WQJ WRC WXSBR XG1 Y6R ZY4 ~KM --- .3N 05W 0R~ 33P 3SF 50Y 50Z 52M 52U 5HH 5LA 5VS 702 8-0 8-3 8UM AAESR AAJUZ AAONW AASGY ABCQN ABCVL ABEML ABHUG ABPVW ACGFO ACPRK ACSCC ADBBV ADKYN ADXAS ADZMN AEGXH AENEX AFRAH AHBTC AIAGR AJXKR ALAGY AUFTA AZBYB AZVAB BAFTC BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BSCLL D-E DPXWK DR2 G.N H.T H.X HZ~ IX1 J0M K48 LC2 LC3 MK4 N04 N05 N9A NF~ O66 O9- P2P P2W P2X Q.N Q11 R.K RX1 V8K W8V W99 WBKPD WNSPC WOHZO WYISQ ~IA ~WT AAHBH AITYG HGLYW OIG NPM AAYXX CITATION 7X8 |
| ID | FETCH-LOGICAL-c4303-41c9dc487f6637c0ecd8327be76382234351b15a508fecf79559df1b03373f523 |
| IEDL.DBID | DR2 |
| ISSN | 1471-8278 1755-098X |
| IngestDate | Sat Aug 17 00:45:14 EDT 2024 Fri Aug 23 03:54:50 EDT 2024 Sat Sep 28 07:48:15 EDT 2024 Sat Aug 24 00:39:37 EDT 2024 Wed Jan 17 05:04:58 EST 2024 Wed Dec 27 19:18:21 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| License | 2007 The Authors. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4303-41c9dc487f6637c0ecd8327be76382234351b15a508fecf79559df1b03373f523 |
| Notes | http://dx.doi.org/10.1111/j.1471-8286.2007.01933.x ArticleID:MEN1933 ark:/67375/WNG-3ZMT18GJ-R istex:DB4B7EB8D397541DD77D8FBA93731285A87F8579 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 21585763 |
| PQID | 867721875 |
| PQPubID | 23479 |
| PageCount | 3 |
| ParticipantIDs | proquest_miscellaneous_867721875 crossref_primary_10_1111_j_1471_8286_2007_01933_x pubmed_primary_21585763 wiley_primary_10_1111_j_1471_8286_2007_01933_x_MEN1933 istex_primary_ark_67375_WNG_3ZMT18GJ_R fao_agris_US201300833590 |
| PublicationCentury | 2000 |
| PublicationDate | 2008 2008-01 January 2008 2008-Jan 2008-01-00 20080101 |
| PublicationDateYYYYMMDD | 2008-01-01 |
| PublicationDate_xml | – year: 2008 text: 2008 |
| PublicationDecade | 2000 |
| PublicationPlace | Oxford, UK |
| PublicationPlace_xml | – name: Oxford, UK – name: England |
| PublicationTitle | Molecular ecology notes |
| PublicationTitleAlternate | Mol Ecol Resour |
| PublicationYear | 2008 |
| Publisher | Oxford, UK : Blackwell Publishing Ltd Blackwell Publishing Ltd |
| Publisher_xml | – name: Oxford, UK : Blackwell Publishing Ltd – name: Blackwell Publishing Ltd |
| References | Marshall TC, Slate J, Kruuk L, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7, 639-655. Ying JS, Zhang YL (1994) Seed Plant Genera Endemic to China. Science Press, Beijing, China. Raymond M, Rousset F (1995) genepop version 1.2: population genetics software for exact tests and ecumenicism. Journal of Heredity, 86, 248-249. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Molecular Ecology, 11, 1-16. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15. 1995; 86 1998; 7 2000 1987; 19 1994 2002; 11 e_1_2_3_2_1 e_1_2_3_7_1 Rozen S (e_1_2_3_5_1) 2000 Ying JS (e_1_2_3_6_1) 1994 e_1_2_3_4_1 e_1_2_3_3_1 |
| References_xml | – start-page: 365 year: 2000 end-page: 386 – volume: 86 start-page: 248 year: 1995 end-page: 249 article-title: genepop version 1.2: population genetics software for exact tests and ecumenicism publication-title: Journal of Heredity – year: 1994 – volume: 19 start-page: 11 year: 1987 end-page: 15 article-title: A rapid DNA isolation procedure for small quantities of fresh leaf tissue publication-title: Phytochemical Bulletin – volume: 7 start-page: 639 year: 1998 end-page: 655 article-title: Statistical confidence for likelihood‐based paternity inference in natural populations publication-title: Molecular Ecology – volume: 11 start-page: 1 year: 2002 end-page: 16 article-title: Strategies for microsatellite isolation: a review publication-title: Molecular Ecology – ident: e_1_2_3_4_1 doi: 10.1093/oxfordjournals.jhered.a111573 – start-page: 365 volume-title: Bioinformatics Methods and Protocols: Methods in Molecular Biology year: 2000 ident: e_1_2_3_5_1 contributor: fullname: Rozen S – volume-title: Seed Plant Genera Endemic to China year: 1994 ident: e_1_2_3_6_1 contributor: fullname: Ying JS – ident: e_1_2_3_3_1 doi: 10.1046/j.1365-294x.1998.00374.x – ident: e_1_2_3_7_1 doi: 10.1046/j.0962-1083.2001.01418.x – ident: e_1_2_3_2_1 doi: 10.1007/s10592-005-9073-x |
| SSID | ssj0017924 ssj0060974 |
| Score | 1.899656 |
| Snippet | Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24... Twenty‐nine primer pairs flanking microsatellite repeats were designed from an AC‐enriched genomic library of Psilopeganum sinense and tested using 24... Abstract Twenty‐nine primer pairs flanking microsatellite repeats were designed from an AC‐enriched genomic library of Psilopeganum sinense and tested using 24... |
| SourceID | proquest crossref pubmed wiley istex fao |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 227 |
| SubjectTerms | conservation endangered species microsatellite microsatellite repeats natural resource management Psilopeganum sinense Rutaceae |
| Title | Isolation and characterization of microsatellite loci in Psilopeganum sinense Hemsl (Rutaceae), an endangered herb endemic to Yangtze River valley |
| URI | https://api.istex.fr/ark:/67375/WNG-3ZMT18GJ-R/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1471-8286.2007.01933.x https://www.ncbi.nlm.nih.gov/pubmed/21585763 https://search.proquest.com/docview/867721875 |
| Volume | 8 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9NAEF1BJSQu0PJVU6j2gBBIOPL6Mz5SlDRUagShEYHLaj-rKI1d1TZq-zP4xZ1ZJxapekCIS5TI8ipev5l5a795S8gbyWIomzL3036ifWDEqS_SBJIhmsPFDBiydWqLcTqaxkezZLbSP2EvTOsP0T1ww8hw-RoDXMjqVpBnzLVBr5wIgYtEPeST6KuH_GjSOUmlQe4MmaFYJn6Q92ebop47B9qoVPetKIG_4tRf3kVGN7mtK07Dx2SxvqxWk7LoNbXsqetbjo__57q3yaMVh6UfW9DtkHumeEIeDJz_9dVT8vsz4NndcCoKTVVnCd12fNLS0iXKACvh3EBrQ6Ggzum8oF-q-Vl5bk5F0SwpCvKLytCRWVZn9N2kqYUywrz_AKNSU2jXsGw0BdBJ_I0qf1qX9AccqK8NnaDihP7CvWKunpHpcHDyaeSvtn7wVQxF1Y-ZyrUC6FhgRJkKjNKQejJpIB0CpYmA5DHJEgH00hplM_TR05bJIIqyyMLi-jnZKsrC7BJqQ5MFOhSxDAGXKhYG5isXoQ6l0rGNPcLWt5mftw4f_M-VUcY4zjXu15lxN9f80iO7gAcuTiER8-m3EF__Bti-lgceeetA0o0lLhYonssS_n18yKOfxyesf3jEJx6haxRxCGl8TyMKUzYVR4vBEMPEIy9adHWDAUHrwwox8kjqMPLX_5gfD8b47eW_nrhHHrZSGXz69Ips1ReNeQ18rJb7LtLgc3jw9QazNyQ_ |
| link.rule.ids | 315,786,790,1382,27955,27956,46327,46751 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtNAFB1BEYINb6jLaxYIgYQjj5_xEkHatDQRCokIbEbzrKImdlXbVdvP4Iu5d5xEpOoCIXaJIo_i8bn3nhmfe4aQN5LFUDZl7qfdRPvAiFNfpAkkQzSHixkwZOvUFsO0P4kPpsl0eRwQ9sK0_hDrDTeMDJevMcBxQ_pKlGfM9UEvrQiBjEQdIJS3IPoTjNLPo7WXVBrkzpIZymXiB3l3uinruXakjVp104oSGCxO_vl1dHST3brytHufzFc31qpSjjtNLTvq8orn43-68wfk3pLG0o8t7h6SG6Z4RG73nAX2xWPyax8g7Z45FYWmau0K3TZ90tLSBSoBK-EMQWtDoabO6KygX6vZvDwxR6JoFhQ1-UVlaN8sqjl9N2pqoYww7z_AqNQU2vUsG00BdxK_o9Cf1iX9AT_Ul4aOUHRCz_C4mIsnZLLbG3_q-8vTH3wVQ131Y6ZyrQA9FkhRpgKjNGSfTBrIiMBqIuB5TLJEAMO0RtkMrfS0ZTKIoiyysL5-SraKsjDbhNrQZIEORSxDgKaKhYH5ykWoQ6l0bGOPsNVz5ietyQf_c3GUMY5zjUd2ZtzNNT_3yDYAgosjyMV88i3EN8ABdrDlgUfeOpSsxxKnx6ifyxL-fbjHo5-DMevuHfCRR-gKRhyiGl_ViMKUTcXRZTDESPHIsxZe68GAo3VhkRh5JHUg-et_zAe9IX7a-dcLX5M7_fHgkB_uD788J3db5QxuRr0gW_VpY14CPavlKxd2vwEpISdl |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtNAFB1BEYgNb6h5zgIhkHDk8TNeIpo0LTSqQiNSNqN5VlEaO6pt1PYz-GLuHScRqbpAiF2syCN7fO69Z-xzzxDyVrIYyqbM_bSbaB8YceqLNIFkiOZwMQOGbJ3aYpgOxvH-JJks9U_YC9P6Q6xfuGFkuHyNAb7Q9kqQZ8y1QS-dCIGLRB3gk7fiNApxIbYzWltJpUHuHJmhWiZ-kHcnm6qea0faKFU3rSiBwOLcn1_HRjfJratO_ftktrqvVpQy6zS17KjLK5aP_-fGH5B7SxJLP7Woe0humOIRud1zBtgXj8mvPQC0e-JUFJqqtSd02_JJS0vnqAOshLMDrQ2Fijql04IeVtPTcmFORNHMKSryi8rQgZlXp_T9qKmFMsJ8-AijUlNo17FsNAXUSTxGmT-tS3oMf9SXho5QckJ_4mYxF0_IuN87-jzwl3s_-CqGqurHTOVaAXYsUKJMBUZpyD2ZNJAPgdNEwPKYZIkAfmmNshka6WnLZBBFWWRhdf2UbBVlYbYJtaHJAh2KWIYATBULA_OVi1CHUunYxh5hq8fMF63FB_9zaZQxjnONG3Zm3M01P_fINuCBixPIxHz8LcTvvwH2r-WBR945kKzHEmczVM9lCf8-3OXRj4Mj1t3d5yOP0BWKOMQ0fqgRhSmbiqPHYIhx4pFnLbrWgwFD68ISMfJI6jDy11fMD3pD_PX8X098Q-4c7vT5173hlxfkbiubwTdRL8lWfdaYV8DNavnaBd1vfYAmFA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Isolation+and+characterization+of+microsatellite+loci+in+Psilopeganum+sinense+Hemsl+%28Rutaceae%29%2C+an+endangered+herb+endemic+to+Yangtze+River+valley&rft.jtitle=Molecular+ecology+resources&rft.au=TANG%2C+FEIYAN&rft.au=YE%2C+QIGANG&rft.au=YAO%2C+XIAOHONG&rft.au=HUANG%2C+HONGWEN&rft.date=2008-01-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=1755-098X&rft.eissn=1755-0998&rft.volume=8&rft.issue=1&rft.spage=227&rft.epage=229&rft_id=info:doi/10.1111%2Fj.1471-8286.2007.01933.x&rft.externalDBID=n%2Fa&rft.externalDocID=ark_67375_WNG_3ZMT18GJ_R |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1471-8278&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1471-8278&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1471-8278&client=summon |