Isolation and characterization of microsatellite loci in Psilopeganum sinense Hemsl (Rutaceae), an endangered herb endemic to Yangtze River valley

• Published in: Molecular ecology notes Vol. 8; no. 1; pp. 227 - 229
• Main Authors: TANG, FEIYAN, YE, QIGANG, YAO, XIAOHONG, HUANG, HONGWEN
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2008; Blackwell Publishing Ltd
• Subjects: conservation; endangered species; microsatellite; microsatellite repeats; natural resource management; Psilopeganum sinense; Rutaceae
• ISSN: 1471-8278; 1755-098X; 1471-8286; 1755-0998
• DOI: 10.1111/j.1471-8286.2007.01933.x

Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from two to eight, with an average value of 3.7. The ranges of observed and expected heterozygosities were 0.000-0.750 and 0.365-0.800, respectively. These microsatellite loci have been directly applied to the ongoing conservation genetic studies of P. sinense.