Roles of long, non-coding RNA in chromosome-wide transcription regulation: Lessons from two dosage compensation systems

• Published in: Biochimie Vol. 94; no. 7; pp. 1490 - 1498
• Main Authors: Maenner, Sylvain, Müller, Marisa, Becker, Peter B.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.07.2012; Elsevier
• Subjects: Animals; Chromatin; Chromosomes - genetics; Dosage compensation; Dosage Compensation, Genetic - genetics; Drosophila; Drosophila melanogaster - genetics; Epigenetic regulation; Gene Expression Regulation - genetics; Histone modifications; Humans; Life Sciences; Long non-coding RNAs; RNA, Untranslated - chemistry; RNA, Untranslated - genetics; Transcription, Genetic - genetics; HOTAIR; MSL1-2-3; Chromatin; ChIRP; lncRNA; SXL; MLE; RXCI; Epigenetic regulation; ES cells; H3K36me3; Suz12; Xi; RIP-chip; RNP; LINE; UNR; Xist; Long non-coding RNAs; Dosage compensation; DCC; Eed; MOF; H3K4me2; Histone modifications; CHART; CES; H4K16Ac; XIC; PRC2; roX; HAS; Ezh2; LNA; DC; H3K27me3; PTB
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2011.12.026

A large part of higher eukaryotic genomes is transcribed into RNAs lacking any significant open reading frame. This “non-coding part” has been shown to actively contribute to regulating gene expression, but the mechanisms are largely unknown. Particularly instructive examples are provided by the dosage compensation systems, which assure that the single X chromosome in male cells and the two X chromosomes in female cells give rise to similar amounts of gene product. Although this is achieved by very different strategies in mammals and fruit flies, long, non-coding RNAs (lncRNAs) are involved in both cases. Here we summarize recent progress towards unraveling the mechanisms, by which the Xist and roX RNAs mediate the selective association of regulators with individual target chromosomes, to initiate dosage compensation in mammals and fruit flies, respectively. ► Xist and roX RNAs are involved in two different dosage compensation systems. ► Xist and roX are very different but exhibit mechanistic similarities. ► Xist and roX recruit chromatin-modifying complexes. ► The function of Xist and roX in dosage compensation depends on their structure. ► Xist and roX structures are likely to be modulated in the process of compensation.