In vitro evaluation of the effect of a high plant protein diet and nucleotide supplementation on intestinal integrity in meagre (Argyrosomus regius)

• Published in: Fish physiology and biochemistry Vol. 39; no. 5; pp. 1365 - 1370
• Main Authors: de Rodrigáñez, M. A. Sáenz, Fuentes, J, Moyano, F. J, Ribeiro, L
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.10.2013; Springer Netherlands; Springer Nature B.V
• Subjects: Absorption; Animal Anatomy; Animal Biochemistry; Animal Feed - analysis; animal growth; Animal Physiology; Animals; Aquaculture; Argyrosomus regius; Biomedical and Life Sciences; Diet; dietary protein; Dietary Supplements; Electric Impedance; Electrolytes; feeds; fish; Freshwater & Marine Ecology; Histology; Ingredients; Intestines - drug effects; Intestines - physiology; Isoquinolines - metabolism; Life Sciences; Membrane Potentials - drug effects; Membrane Potentials - physiology; Morphology; nucleotides; Nucleotides - pharmacology; Patch-Clamp Techniques; Pathogens; Perciformes - growth & development; Permeability; protective effect; Proteins; Sodium; Vegetable Proteins - pharmacology; vegetables; Zoology; United States > US; Portugal; Spain; Nucleotide; Plant meal; USSING chamber; Feed fish; Meagre; Intestinal integrity
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-013-9790-x

In the present study, the protective effect of nucleotides over damages induced by the consumption of a diet containing a high amount of vegetable ingredients (560 g kg⁻¹) in the intestinal epithelia of the meagre (Argyrosomus regius) was assessed by assays performed with an Ussing-type chamber. Two experimental feeds were prepared including or not a commercial mixture of nucleotides (1 g kg⁻¹). Nucleotides significantly enhanced fish growth during the experiment. On the other hand, differences in the integrity and functionality of intestinal epithelia were evidenced by a change in the polarity of intestinal trans-epithelial potential. Samples of fish fed on the control diet showed a preferentially secretory short-circuit current, while those of fish receiving the nucleotide-supplemented diet showed a significantly lower and preferentially absorptive negative current. It is concluded that alterations of intestinal physiology juvenile meagre resulting from the intake of high amounts of plant ingredients could be minimized by nucleotide supplementation.