A zona pellucida 3 peptide vaccine induces antibodies and reversible infertility without ovarian pathology

• Published in: The Journal of immunology (1950) Vol. 155; no. 5; pp. 2715 - 2720
• Main Authors: Lou, Y, Ang, J, Thai, H, McElveen, F, Tung, KS
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.09.1995
• Subjects: Amino Acid Sequence; Animals; Autoantibodies - immunology; Autoimmunity; Egg Proteins - adverse effects; Egg Proteins - immunology; Female; H-2 Antigens - genetics; Immunization; Infertility, Female - chemically induced; Litter Size - immunology; Membrane Glycoproteins - adverse effects; Membrane Glycoproteins - immunology; Mice; Mice, Inbred A; Mice, Inbred C57BL; Mice, Inbred Strains; Molecular Sequence Data; Ovary - pathology; Receptors, Cell Surface; Recombinant Fusion Proteins - immunology; Vaccines, Synthetic - adverse effects; Zona Pellucida Glycoproteins
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.155.5.2715

Zona pellucida 3 (ZP3) is a major glycoprotein of the zona pellucida that possesses the sperm receptor function. ZP3 induces autoantibody that can block sperm/oocyte interaction. However, the feasibility of a ZP3 contraceptive vaccine has been marred by the finding that ZP3-specific T cells mediate ovarian autoimmune disease. Moreover, as reported in this work, only some inbred mouse strains respond to the ZP3 peptide. We now describe a chimeric peptide that induces Abs to native ZP3 regardless of the MHC haplotype of the inbred mice tested. Study in one mouse strain resulted in reduction in fertility that correlates well with zona pellucida Ab titer, and most importantly, the mice do not develop concomitant autoimmune oophoritis. Moreover, the infertility was completely reversible. The design of the vaccine chimeric peptide is governed by the inclusion of two essential components: 1) a promiscuous foreign T cell peptide capable of eliciting a Th cell response regardless of the MHC haplotype of the animals, and 2) the native B cell peptide of ZP3 that has been modified by substitution of residue(s) critical for T cell but not B cell response to ZP3.