Study of quality markers of antiuric acid formula by grey relational analysis

• Published in: SN applied sciences Vol. 3; no. 6; pp. 661 - 10
• Main Authors: Wang, Fang, Cao, Jing, Li, Yang, Ren, Fugang, Bai, Jing, Dong, Qiang, Guo, Jinlin
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2021; Springer Nature B.V; Springer
• Subjects: Antiuric acid formula; Applied and Technical Physics; Biological activity; Chemistry/Food Science; Chemistry: Pharmaceutical; Chinese medicine; Chromatography; Content analysis; Drug dosages; Earth Sciences; Engineering; Environment; Experiments; Fingerprint; Glucosides; Gout; High performance liquid chromatography; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Materials Science; Medical; Medical research; Medicinal and Clinical Chemistry; Metabolism; Orbitrap LC–MS; Pharmacodynamics; Quality control; Reference materials; Research Article; Research methodology; Rheumatism; Scientific imaging; Spectrum–effect analysis; Uric acid; Xanthine oxidase; Beijing China; China; Fingerprint; Pharmacodynamics; Spectrum–effect analysis; Antiuric acid formula; Orbitrap LC–MS
• ISSN: 2523-3963; 2523-3971
• DOI: 10.1007/s42452-021-04248-6

Gout has become a global problem, antiuric acid formula (AAF) is a clinical prescription highly effective in reducing uric acid levels. In order to find its quality control standards and contribute to the treatment of gout in the future, we adopted high-performance liquid chromatography and orbitrap liquid chromatography–mass spectrometry to establish fingerprints of 13 batches of AAF. The different batches of AAF were tested the activity of inhibit uric acid by the xanthine oxidase inhibition experiment. Grey relational analysis and bio-activity validation to assess the spectrum–effect relationship. Finally, we choose puerarin, calycosin-7- O -beta- d -glucoside and puerarin apioside as the AAF quality control component, and its average content is 6036.006 μg/g, 296.113 μg/g and 878.285 μg/g. As the quality control components of AAF, puerarin, calycosin-7- O -beta- d -glucoside and puerarin apioside can be of great significance for the treatment of gout and gout related research.