Structural investigation of two neutral polysaccharides isolated from rhizome of Polygonatum sibiricum

• Published in: Carbohydrate polymers Vol. 70; no. 3; pp. 304 - 309
• Main Authors: Liu, Liu, Dong, Qun, Dong, Xiao-tang, Fang, Ji-nian, Ding, Kan
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2007; Elsevier Science
• Subjects: Applied sciences; bioactive properties; Biological and medical sciences; chemical composition; Chemical constitution; chemical structure; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Galactan; galactans; Galactomannan; galactomannans; herbal medicines; methylation; molecular weight; Natural polymers; oxidation; Physicochemistry of polymers; Plant physiology and development; Polygonatum; Polygonatum sibiricum; Polysaccharide; polysaccharides; rhizomes; roots; Starch and polysaccharides; traditional medicine; Polygonatum sibiricum; Galactomannan; Galactan; Polysaccharide; Monocotyledones; Branched polymer; Molecular structure; Chemical isolation; Experimental study; Solvent extraction; Medicinal plant; Liliaceae; Chinese medicine; Folk medicine; Angiospermae; Plant origin; Rhizome; Oside polymer; Spermatophyta
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2007.04.012

Two neutral polysaccharides, PSW-1a and PSW-1b-2, were isolated from the hot-water extract of Polygonatum sibiricum roots, using ethanol precipitation, anion-exchange chromatography, Fehling reagent complexing, and gel permeation chromatography. Their structures were investigated using composition and linkage analyses, periodate oxidation and Smith degradation, partial acid hydrolysis, and NMR spectroscopic methods. PSW-1b-2 was characterized as a branched homogalactan, containing a 1,4-linked β- d-galactopyranosyl backbone with one β- d-galactopyranosyl stub substituted at O-6 of every 7th backbone residue, whereas PSW-1a as a highly branched galactomannan, possessing a 1,4-linked β- d-mannopyranosyl backbone, with a β- d-galactopyranosyl stub attached to O-6 of every 9th mannosyl residue.