Effect of Actin Polymerization Inhibitor During Oocyte Maturation on Parthenogenetic Embryo Development and Ploidy in Capra hircus

• Published in: Biochemical genetics Vol. 51; no. 11-12; pp. 944 - 953
• Main Authors: Ranjan, R, Singh, R. K, Yasotha, T, Kumar, Manish, Puri, Gopal, Kumar, Kuldeep, Singh, Renu, Bhure, Sanjeev, Malakar, D, Bhanja, S. K, Sarkar, M, Das, B. C, Bag, Sadhan
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.12.2013; Springer US; Springer Nature B.V
• Subjects: actin; Actins - metabolism; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Capra hircus; cytochalasin B; Cytochalasin B - pharmacology; early development; embryogenesis; Embryonic Development - drug effects; Embryonic Development - genetics; Embryos; Ethanol; Female; Fertilization in Vitro; goats; Goats - embryology; Goats - genetics; Human Genetics; Karyotype; Medical Microbiology; oocytes; Oocytes - drug effects; Oocytes - growth & development; parthenogenesis; Parthenogenesis - genetics; Ploidies; ploidy; polymerization; Zoology; DMAP; CCB; Diploid parthenogenetic embryo; Karyotyping; Caprine
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-013-9619-4

This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.