A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth

has attracted tremendous interest owing to its compelling health-promoting properties. However, breeding of elite cultivars of is hindered by the lack of a genetic and molecular toolbox. Here, we performed resequencing analysis of 127 F single-spore isolates and constructed the first high-resolution...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in microbiology Vol. 10; p. 3129
Main Authors Gong, Wenbing, Xie, Chunliang, Zhou, Yingjun, Zhu, Zuohua, Wang, Yahui, Peng, Yuande
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 31.01.2020
Subjects
Hericium erinaceus
high-resolution bin map
Microbiology
monokaryon growth
quantitative trait loci
resequencing
monokaryon growth
quantitative trait loci
resequencing
Hericium erinaceus
high-resolution bin map
Online AccessGet full text

Cover

More Information
Summary:has attracted tremendous interest owing to its compelling health-promoting properties. However, breeding of elite cultivars of is hindered by the lack of a genetic and molecular toolbox. Here, we performed resequencing analysis of 127 F single-spore isolates and constructed the first high-resolution genetic map of . With the use of recombination bins as markers, an ultradense genetic map consisting of 1,174 bins (including 37,082 single-nucleotide polymorphisms) was generated. This newly developed genetic map covered 1,096.5 cM, with an average bin spacing of 0.95 cM. High collinearity between genetic map and genome assembly was revealed by aligning scaffolds to this genetic map using bin markers as anchors. The application of this newly developed genetic map in quantitative trait locus (QTL) mapping was also elucidated, and four QTLs for monokaryon growth were recovered. One QTL, , which contributes 12.1% of growth variations, was located near the mating type A (MAT-A) loci. Overall, this newly constructed high-resolution genetic map (or bin map) could be used as reference in future genetic, genomic, and breeding studies on .
Bibliography:Edited by: Mohamed Hijri, Université de Montréal, Canada
This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology
Reviewed by: Chenyang Huang, Institute of Agricultural Resources and Regional Planning (CAAS), China; Matt Anderson, The Ohio State University, United States
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.03129