Yessotoxin triggers ribotoxic stress

• Published in: Toxicology in vitro Vol. 28; no. 5; pp. 975 - 981
• Main Authors: Korsnes, Mónica Suárez, Røed, Susan Skogtvedt, Tranulis, Michael A., Espenes, Arild, Christophersen, Berit
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2014
• Subjects: 28S rRNA cleavage; Animals; c-jun; Cell Line; eIF-2 Kinase - metabolism; JNK Mitogen-Activated Protein Kinases - metabolism; Marine Toxins - toxicity; Mice; Oxocins - toxicity; p38 Mitogen-Activated Protein Kinases - metabolism; PKR; Protein Biosynthesis - drug effects; Protein synthesis inhibition; Rats; Ribotoxic stress response; RNA Cleavage; RNA, Ribosomal, 28S - metabolism; Yessotoxin; c-jun; 28S rRNA cleavage; Yessotoxin; PKR; Ribotoxic stress response; Protein synthesis inhibition
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2014.04.013

•Yessotoxin can act as a ribotoxin.•It triggers ribotoxic stress in L6 and BC3H1 cells.•It also causes ribosomal RNA cleavage and protein synthesis inhibition. This work tests the hypothesis that the marine algal toxin yessotoxin (YTX) can trigger ribotoxic stress response in L6 and BC3H1 myoblast cells. YTX exposure at a concentration of 100nM displays the characteristics of a ribotoxic stress response in such cells. The exposure leads to activation of the p38 mitogen-activated protein kinase, the stress-activated protein kinase c-jun, and the double-stranded RNA-activated protein kinase (PKR). YTX treatment also causes ribosomal RNA cleavage and inhibits protein synthesis. These observations support the idea that YTX can act as a ribotoxin.