Kallikrein Activates Prothrombin

Kallikrein is a multitalented enzyme in hemostasis and inflammation. Normally, kallikrein is formed in intrinsic hemostasis and activates factor XII. A total of 10 µL of 0 to 100 µg/mL human plasma kallikrein in 6% human albumin—PBS were incubated with 90 µL 111.1 µg/mL prothrombin in 6% human album...

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Bibliographic Details
Published inClinical and applied thrombosis/hemostasis Vol. 14; no. 1; pp. 97 - 98
Main Author Stief, Thomas W.
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.01.2008
SAGE PUBLICATIONS, INC
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Summary:Kallikrein is a multitalented enzyme in hemostasis and inflammation. Normally, kallikrein is formed in intrinsic hemostasis and activates factor XII. A total of 10 µL of 0 to 100 µg/mL human plasma kallikrein in 6% human albumin—PBS were incubated with 90 µL 111.1 µg/mL prothrombin in 6% human albumin in absence and presence of 23 mM Ca++. After 0 to 64 minutes (37°C), 100 µL of 2.5 M arginine, pH 9, were added. Fifty microliters of 0.72 mM HD-CHG-Ala-Arg-pNA in 1.36 M arginine were added and increase in absorbance at 405 nm was determined. Within 8 minutes (37°C), 1 µg/mL kallikrein, ie, 2.5% of the normal plasmatic prekallikrein concentration, generates approximately 3 mIU/mL thrombin in absence and 27 mIU/mL thrombin in presence of Ca++. Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway.
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ISSN:1076-0296
1938-2723
DOI:10.1177/1076029607308036