Complete genome sequence of a Watermelon silver mottle virus isolate from China

• Published in: Virus genes Vol. 46; no. 3; pp. 576 - 580
• Main Authors: Rao, Xueqin, Wu, Zhuyan, Li, Yuan
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.06.2013; Springer US; Springer Nature B.V
• Subjects: amino acid sequences; Biomedical and Life Sciences; Biomedicine; Bunyaviridae; China; Citrullus - virology; Cluster Analysis; DNA-directed RNA polymerase; Gene Order; genome; Genome, Viral; glycoproteins; Medical Microbiology; Molecular Sequence Data; nucleocapsid; nucleotide sequences; Phylogeny; Plant Diseases - virology; Plant Sciences; RNA; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tospovirus; Tospovirus - genetics; Tospovirus - isolation & purification; Viral Proteins - genetics; Virology; Watermelon silver mottle virus; China; WSMoV; Watermelon; Sequence analysis
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-013-0885-3

The complete genome of a Watermelon silver mottle virus (WSMoV) (genus Tospovirus, family Bunyaviridae) isolate (WSMoV-GZ) from Guangdong province, China was sequenced. The genomes of WSMoV-GZ contained 3,603, 4,909, and 8,914 nt of small (S), medium (M), and large (L) RNA segments, respectively, and had a genomic organization characteristic of members of the genus Tospovirus. The amino acid sequence of the nucleocapsid (N) protein, S RNA-encoded nonstructural (NSs) protein, M RNA-encoded nonstructural (NSm) protein, Gn/Gc glycoprotein precursor, and RNA-dependent RNA polymerase (RdRp) protein showed 94.3–97.5 % identity with those of other WSMoV isolates. Phylogenetic analysis showed that the N protein of WSMoV-GZ was clustered together with those of the WSMoV isolates. The full sequence of WSMoV-GZ provides a reference genome for comparison with other tospoviruses.