Antimicrobial susceptibility of microflora from ovine cheese

• Published in: Folia microbiologica Vol. 57; no. 4; pp. 291 - 293
• Main Authors: Kme, V, Drugdová, Z
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.07.2012; Springer Netherlands; Springer Nature B.V
• Subjects: Ampicillin; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Applied Microbiology; Bacteria; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; beta -Lactamase; Biomedical and Life Sciences; Cheese; Cheese - microbiology; cheeses; Clindamycin; Dairy products; desorption; E coli; Enrofloxacin; Environmental Engineering/Biotechnology; Escherichia coli; Florfenicol; Gentamicin; Immunology; Ionization; Lactic acid bacteria; Lactobacillus brevis; Lactobacillus casei; Lactobacillus delbrueckii; Lactobacillus fermentum; Lactobacillus helveticus; Lactobacillus paracasei; Lactobacillus plantarum; Lactobacillus rhamnosus; Lactococcus lactis; Lasers; Life Sciences; Microbial Sensitivity Tests; Microbiology; Microflora; Milk; Pediococcus acidilactici; Pediococcus pentosaceus; Sheep; Streptomycin; tetracycline; Tetracyclines; Minimal Inhibitory Concentration; Tigecycline; Daptomycin; Lactobacillus; Lactic Acid Bacterium
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-012-0128-3

Strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included Lactobacillus casei/Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus brevis, Lactococcus lactis, Pediococcus pentosaceus and Pediococcus acidilactici. The susceptibility toward antibiotics was determined in lactobacilli, lactococci and pediococci and also in Escherichia coli for comparison. Analysis of L. fermentum and pediococci revealed the presence of non-wild-type epidemiological cut-offs in streptomycin, clindamycin or gentamicin. E. coli were resistant to ampicillin, tetracycline, enrofloxacin and florfenicol. No extended spectrum β-lactamases were detected.