Comparison of Stenocarpella maydis isolates for isozyme and cultural characteristics

Isolates of Stenocarpella maydis from seed companies and plant disease clinics in the United States and the Republic of South Africa were assayed for isozyme polymorphisms and cultural variability. A low level of isozyme polymorphisms was detected in this collection of isolates. Isozyme polymorphism...

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Bibliographic Details
Published inPlant disease Vol. 83; no. 7; pp. 675 - 680
Main Authors Dorrance, A.E, Miller, O.K, Warren, H.L
Format Journal Article
LanguageEnglish
Published United States 01.07.1999
Subjects
ambient temperature
color
comparisons
cultured cells
enzyme polymorphism
fungal spores
genetic markers
genetic variation
growth
host plants
host range
isozymes
phenotype
plant diseases and disorders
sporulation
Stenocarpella maydis
strain differences
United States
South Africa
Diplodia ear rot
maize
Diplodia stalk rot
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Summary:Isolates of Stenocarpella maydis from seed companies and plant disease clinics in the United States and the Republic of South Africa were assayed for isozyme polymorphisms and cultural variability. A low level of isozyme polymorphisms was detected in this collection of isolates. Isozyme polymorphisms were detected for alpha-esterase, hexose kinase, and malate dehydrogenase of the enzymes assayed. Fungi often have limited variability among isozyme profiles, and this is especially true for fungi that have host specialization such as biotrophs or fungi with formae speciales designations. Optimum growth temperature, colony color, and pycnidiospore production were also measured. All isolates had an optimum temperature of 28 to 31 degrees C for colony growth on acidified potato dextrose agar. Colony color and pycnidiospore production were variable over the course of several experiments, indicating that these phenotypes are poor genetic markers.
ISSN:0191-2917
1943-7692
DOI:10.1094/PDIS.1999.83.7.675