Short Communication: Characterization of a New Genetic Variant in the Caprine k-casein Gene
A new polymorphism has been identified in the goat k-casein gene by evaluating genomic DNA from the Montefalcone breed in Italy. The polymorphic site consists of a single nucleotide substitution A to G at position 242 of the exon 4 and produces an amino acid substitution Asp/Gly. A polymerase chain...
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Published in | Journal of dairy science Vol. 85; no. 10; pp. 2679 - 2680 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Champaign
Elsevier Inc
01.10.2002
Am Dairy Sci Assoc American Dairy Science Association |
Subjects | |
Online Access | Get full text |
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Summary: | A new polymorphism has been identified in the goat k-casein gene by evaluating genomic DNA from the Montefalcone breed in Italy. The polymorphic site consists of a single nucleotide substitution A to G at position 242 of the exon 4 and produces an amino acid substitution Asp/Gly. A polymerase chain reaction-restriction fragment length polymorphism protocol for rapid genotyping of the variant has been developed, using the HaeIII enzyme. Animals from Italian, Spanish, and French breeds have been analyzed to investigate the occurrence of the allele in other populations. The allele appears to be exclusive to the Montefalcone breed. |
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ISSN: | 0022-0302 1525-3198 |
DOI: | 10.3168/jds.S0022-0302(02)74353-1 |