Quantitation of virus using laser-based scanning of near-infrared fluorophores replaces manual plate reading in a virus titration assay

A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The im...

Full description

Saved in:
Bibliographic Details
Published inJournal of virological methods Vol. 168; no. 1; pp. 57 - 62
Main Authors Weldon, Sally K., Mischnick, Shawn L., Urlacher, Teresa M., Ambroz, Kristi L.H.
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier B.V 01.09.2010
Elsevier
Subjects
Animals
Automation - methods
Biological and medical sciences
BVDV
Cell Line
Computer programs
Feline Panleukopenia Virus - isolation & purification
FPV
Fundamental and applied biological sciences. Psychology
Infectivity assay
Lasers
Microbiology
Near-infrared fluorescence
Odyssey
Techniques used in virology
Viral Load - methods
Virology
Odyssey
BVDV
Infectivity assay
FPV
Near-infrared fluorescence
Infectivity
Method
Quantitative analysis
Online AccessGet full text

Cover

More Information
Summary:A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The image data is translated by LI-COR's Odyssey software into numerical data which is used directly in the virus titer calculations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2010.04.016