A Feline Herpesvirus-1 Recombinant with a Deletion in the Genes for Glycoproteins gI and gE Is Effective as a Vaccine for Feline Rhinotracheitis

• Published in: Virology (New York, N.Y.) Vol. 214; no. 1; pp. 12 - 20
• Main Authors: SUSSMAN, MICHAEL D., MAES, ROGER K., KRUGER, JOHN M., SPATZ, STEPHEN J., VENTA, PATRICK J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.1995
• Subjects: Alphaherpesvirinae - genetics; Alphaherpesvirinae - immunology; Animals; Base Sequence; beta-Galactosidase - genetics; Cat Diseases - immunology; Cat Diseases - prevention & control; Cat Diseases - virology; Cats; Cell Line; CHAT; DNA Primers; DNA, Viral; GATO; GENE; Gene Deletion; GENES; GLICOPROTEINAS; GLYCOPROTEINE; Glycoproteins - genetics; Herpesviridae Infections - immunology; Herpesviridae Infections - prevention & control; Herpesviridae Infections - veterinary; HERPETOVIRIDAE; Molecular Sequence Data; MUTACION; MUTATION; Tracheitis - immunology; Tracheitis - prevention & control; Tracheitis - veterinary; Tracheitis - virology; VACCIN SYNTHETIQUE; VACCINATION; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - genetics; VACUNA SINTETICA; VACUNACION; Viral Proteins - genetics; Viral Vaccines - administration & dosage; Viral Vaccines - genetics
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1006/viro.1995.9959

Using a site-directed mutagenesis technique we constructed a new feline herpesvirus-1 recombinant strain containing a deletion in two genes encoding glycoproteins gI and gE. These proteins may have a role in virulence, the establishment of latency, and viral recurrence as shown in other herpesviruses of the varicella and simplex types. This recombinant was characterized and used to immunize juvenile cats against virulent virus challenge. Significant protection resulted from vaccination of cats by the subcutaneous route.