Colorimetric assay of l-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters
This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l-cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for l-cysteine was developed with a detection limit (LOD) of 2.0nM and a linear range fro...
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| Published in | Sensors and actuators. B, Chemical Vol. 235; pp. 110 - 116 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
01.11.2016
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| Subjects | |
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| Abstract | This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l-cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for l-cysteine was developed with a detection limit (LOD) of 2.0nM and a linear range from 5.0nM to 500nM. This detection for l-cysteine was highly selectivity over other amino acids. The high sensitivity and selectivity of this method facilitated the detection of l-cysteine in biological samples. Moreover, this method developed here was low-cost and simple. These results indicated the excellent analytical application of Ag/Pt NCs as enzyme mimic. |
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| AbstractList | This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l-cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for l-cysteine was developed with a detection limit (LOD) of 2.0nM and a linear range from 5.0nM to 500nM. This detection for l-cysteine was highly selectivity over other amino acids. The high sensitivity and selectivity of this method facilitated the detection of l-cysteine in biological samples. Moreover, this method developed here was low-cost and simple. These results indicated the excellent analytical application of Ag/Pt NCs as enzyme mimic. |
| Author | Wang, Li-Ying Peng, Chi-Fang Wu, Liang-Liang Xie, Zheng-Jun Pan, Na |
| Author_xml | – sequence: 1 givenname: Liang-Liang surname: Wu fullname: Wu, Liang-Liang – sequence: 2 givenname: Li-Ying surname: Wang fullname: Wang, Li-Ying – sequence: 3 givenname: Zheng-Jun surname: Xie fullname: Xie, Zheng-Jun – sequence: 4 givenname: Na surname: Pan fullname: Pan, Na – sequence: 5 givenname: Chi-Fang surname: Peng fullname: Peng, Chi-Fang email: pcf@jiangnan.edu.cn |
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| Snippet | This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l-cysteine. Based on this finding, a... |
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| SubjectTerms | Actuators Ag/Pt nanoclusters Amino acids Biological properties Colorimetric detection Colorimetry Enzyme mimic l-Cysteine Mathematical analysis Nanostructure Platinum Selectivity |
| Title | Colorimetric assay of l-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters |
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