Isolation and characterization of a bacteriophage with broad host range, displaying potential in preventing bovine diarrhoea

Phage therapy has been previously tried for treatment of diarrhoea in calves, pigs and lambs but those trials were conducted without any detailed information of used phages. Here, we report isolation of a broad-spectrum phage which showed bactericidal activity against 47.3 % of calf diarrhoeal isola...

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Bibliographic Details
Published inVirus genes Vol. 51; no. 2; pp. 315 - 321
Main Authors Anand, Taruna, Vaid, Rajesh K., Bera, Bidhan Ch, Barua, Sanjay, Riyesh, T., Virmani, N., Yadav, Neeraj, Malik, Praveen
Format Journal Article
LanguageEnglish
Published New York Springer US 01.10.2015
Springer Nature B.V
Subjects
Animals
Bacteriophages - isolation & purification
Bacteriophages - physiology
Bacteriophages - ultrastructure
Biological Therapy - methods
Biomedical and Life Sciences
Biomedicine
Cattle
Cattle Diseases - prevention & control
Diarrhea - prevention & control
Diarrhea - veterinary
DNA, Viral - chemistry
DNA, Viral - genetics
Escherichia coli
Escherichia coli Infections - prevention & control
Escherichia coli Infections - veterinary
Host Specificity
Medical Microbiology
Microscopy, Electron, Transmission
Molecular Sequence Data
Myoviridae
Myoviridae - isolation & purification
Myoviridae - physiology
Myoviridae - ultrastructure
Plant Sciences
Sequence Analysis, DNA
Sequence Homology
Viral Nonstructural Proteins - genetics
Virion - ultrastructure
Virology
Diarrhoea
Bacteriophage
Electron microscopy
Myoviridae
E. coli
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Summary:Phage therapy has been previously tried for treatment of diarrhoea in calves, pigs and lambs but those trials were conducted without any detailed information of used phages. Here, we report isolation of a broad-spectrum phage which showed bactericidal activity against 47.3 % of calf diarrhoeal isolates of Escherichia coli, in vitro. The isolated phage resembled the characteristics of Myoviridae family and showed ~97 % similarity with earlier reported bacteriophages of sub family-Tevenvirinae, genus-T4-like virus, based on nucleotide sequence of major head protein—gp23 gene. The phage exhibits the potential to be used as drug substitute tool against E. coli causing diarrhoea in cattle in farm environments.
Bibliography:http://dx.doi.org/10.1007/s11262-015-1222-9
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ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-015-1222-9