Fabrication of Magnetic CLEA-protease Nanocomposite: High Progression in Biotechnology and Protein Waste Management

• Published in: Catalysis letters Vol. 149; no. 7; pp. 1753 - 1764
• Main Authors: Hashemabadi, Mohammad, Badoei-Dalfard, Arastoo
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2019; Springer; Springer Nature B.V
• Subjects: Abattoirs; Analysis; Biotechnology; Catalysis; Chemistry; Chemistry and Materials Science; Economic conditions; Environmental impact; Industrial applications; Industrial Chemistry/Chemical Engineering; Nanocomposites; Organometallic Chemistry; Physical Chemistry; Protease; Proteases; Proteins; Refuse and refuse disposal; Technology application; Washing; Waste management; Wastewater; Yogurt; Immobilization; Protease; Nanocomposite
• ISSN: 1011-372X; 1572-879X
• DOI: 10.1007/s10562-019-02751-5

Magnetic nanocomposite opens ample opportunities for large scale application in industrial biocatalyst market. In this study, we isolated a high potent protease producing Bacillus cereus from several slaughterhouse wastes located in Kerman province, Iran. Results showed that, it was an organic solvent tolerant, thermophile protease which showed 20% increase in protease stability in the presence of n-hexane. It was active in a wide range of pHs and temperatures. A magnetic CLEA-protease nanocomposite (mCLEA-P-NC) was constructed successfully which confirmed by SEM and FTIR analysis. mCLEA-P-NC displayed K m value 4.6 folds lower than free enzyme. Biotechnological applications showed nearly 44% of proteins which obtained from waste water of local yogurt were degraded by mCLEA-P-NC. In addition, it showed high potential in silver recovery and de-haring process. A simple mechanic system was manifested to use mCLEA-P-NC in washing performance. These results indicated high potential of this protease nanocomposite in biotechnological application especially in recycling of waste proteins and washing performance. Graphical Abstract