Fabrication of Magnetic CLEA-protease Nanocomposite: High Progression in Biotechnology and Protein Waste Management
Magnetic nanocomposite opens ample opportunities for large scale application in industrial biocatalyst market. In this study, we isolated a high potent protease producing Bacillus cereus from several slaughterhouse wastes located in Kerman province, Iran. Results showed that, it was an organic solve...
Saved in:
Published in | Catalysis letters Vol. 149; no. 7; pp. 1753 - 1764 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.07.2019
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Magnetic nanocomposite opens ample opportunities for large scale application in industrial biocatalyst market. In this study, we isolated a high potent protease producing
Bacillus cereus
from several slaughterhouse wastes located in Kerman province, Iran. Results showed that, it was an organic solvent tolerant, thermophile protease which showed 20% increase in protease stability in the presence of n-hexane. It was active in a wide range of pHs and temperatures. A magnetic CLEA-protease nanocomposite (mCLEA-P-NC) was constructed successfully which confirmed by SEM and FTIR analysis. mCLEA-P-NC displayed
K
m value 4.6 folds lower than free enzyme. Biotechnological applications showed nearly 44% of proteins which obtained from waste water of local yogurt were degraded by mCLEA-P-NC. In addition, it showed high potential in silver recovery and de-haring process. A simple mechanic system was manifested to use mCLEA-P-NC in washing performance. These results indicated high potential of this protease nanocomposite in biotechnological application especially in recycling of waste proteins and washing performance.
Graphical Abstract |
---|---|
ISSN: | 1011-372X 1572-879X |
DOI: | 10.1007/s10562-019-02751-5 |