Genetic and epistatic effects for grain quality and yield of three grain-size QTLs identified in brewing rice (Oryza sativa L.)

• Published in: Molecular breeding Vol. 40; no. 9
• Main Authors: Okada, Satoshi, Iijima, Ken, Hori, Kiyosumi, Yamasaki, Masanori
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2020; Springer Nature B.V
• Subjects: Abdomen; Alcoholic beverages; Biomedical and Life Sciences; Biotechnology; Brewing; Cooking; Crop yield; Cultivars; Epistasis; Gene mapping; Grain size; Life Sciences; Molecular biology; Oryza sativa; Particle size; Plant biology; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Quantitative trait loci; Rice; Sake; Shape effects; Weight; Rice yield; QTL; Pyramiding line; Epistasis; Grain shape; Grain quality
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-020-01166-0

Rice ( Oryza sativa L.) in Japan is not only a food staple but also an important material for the Japanese alcoholic beverage, sake . The grain used in sake brewing has different characters from the cooking rice grain, including a large grain size and high white-core expression rate (WCE). Because large-sized grains often have a heavy grain weight and higher yield, this trait is also important for cooking rice. Chalky grains, such as white-core or white-belly grains, are not ideal as cooking rice. Here, we report that three grain-size quantitative trait loci (QTLs; qGL4-2 , qGWh5 , qGWh10 ), derived from the brewing cultivar, Yamadanishiki, affect grain shape, chalky grain rate, and yield, using near isogenic and pyramiding lines in the genetic background of the cooking cultivar, Koshihikari. First, these QTLs influenced multiple components of grain shape, where epistatic effects were detected between qGL4-2 and qGWh5 , for grain width and thickness, and between qGL4-2 and qGWh10 , for grain length. Therefore, these QTLs may coordinate to control grain shape. Second, lines harboring qGWh5 or qGWh10 at the Yamadanishiki allele exhibited increased WCE, whereas lines with qGL4-2 and qGWh10 exhibited decreased white-belly grain rate (WBR). Thus, grain shape is associated with the occurrence of chalky grain, where the chalky type depends on the QTL. Finally, we used total panicle weight of plants as a simplified rice yield index, and a promising line pyramiding qGL4-2 and qGWh5 emerged. In conclusion, qGL4-2 would be useful for the breeding of cooking rice, to decrease WBR, while qGWh5 and qGWh10 were definitely more beneficial for that of brewing rice, to increase grain weight and WCE.