Differentiation and characterization of embryonic stem cells into three germ layers

Embryonic stem cells differentiated on M15 cells have previously been shown to give rise to cells of the mesendodermal and definitive endodermal lineages. Here we demonstrate that neuroectodermal and mesodermal lineages can be derived from ES cells cultured on M15 cells and subsequently subjected to...

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Published inBiochemical and biophysical research communications Vol. 381; no. 4; pp. 694 - 699
Main Authors Shiraki, Nobuaki, Higuchi, Yuichiro, Harada, Seiko, Umeda, Kahoko, Isagawa, Takayuki, Aburatani, Hiroyuki, Kume, Kazuhiko, Kume, Shoen
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 17.04.2009
Subjects
Animals
Cell Differentiation
Cell Line
Cell Separation
Embryonic Stem Cells - cytology
Endoderm
ES cells
Germ Layers - cytology
Growth factors
In vitro differentiation
Mesoderm
Mice
Neuroectoderm
Pancreatic differentiation
Neuroectoderm
In vitro differentiation
Mesoderm
ES cells
Pancreatic differentiation
Growth factors
Endoderm
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Summary:Embryonic stem cells differentiated on M15 cells have previously been shown to give rise to cells of the mesendodermal and definitive endodermal lineages. Here we demonstrate that neuroectodermal and mesodermal lineages can be derived from ES cells cultured on M15 cells and subsequently subjected to specific culture conditions, as confirmed by the expression of molecular markers. Prospective isolation and microarray analyses showed that neuroectodermal cells expressed anterior-to-posterior, as well as dorso-ventral regional markers, suggesting that this procedure could be used for the induction of cells belonging to a wide variety of neural lineages. Lateral mesoderm and paraxial mesoderm cells were also produced and their gene expression profiles were confirmed by microarray analyses. These results indicate that the M15 cell system provides a valuable tool for generating ES cell-derived lineage-specific cell types belonging to the three germ layers, namely neuroectoderm, mesoderm, and definitive endoderm.
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ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.02.120