Serological survey of avian influenza virus infection in non-avian wildlife in Xinjiang, China

• Published in: Archives of virology Vol. 161; no. 4; pp. 867 - 872
• Main Authors: Wei, Yu-Rong, Yang, Xue-Yun, Li, Yuan-Guo, Wei, Jie, Ma, Wen-Ge, Ren, Zhi-Guang, Guo, Hui-Ling, Wang, Tie-Cheng, Mi, Xiao-Yun, Adili, Gulizhati, Miao, Shu-Kui, Shaha, Ayiqiaolifan, Gao, Yu-Wei, Huang, Jiong, Xia, Xian-Zhu
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2016; Springer Nature B.V
• Subjects: Animal sciences; Animals; Animals, Wild; Antibodies; Avian flu; Avian influenza virus; Biomedical and Life Sciences; Biomedicine; Bird migration; Bos grunniens; Canis lupus; Capra ibex; Capreolus; Capreolus pygargus; Cervus elaphus; China - epidemiology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Epidemics; Gazella subgutturosa; hemagglutination; Infections; Infectious Diseases; Influenza A virus; Influenza A virus - classification; Influenza A virus - isolation & purification; Marine mammals; Medical Microbiology; Original Article; Orthomyxoviridae Infections - epidemiology; Orthomyxoviridae Infections - veterinary; Orthomyxoviridae Infections - virology; Ovis ammon; Poultry; Predation; Pseudois nayaur; Seroepidemiologic Studies; serological surveys; Serology; seroprevalence; Sus scrofa; Veterinary medicine; Virology; Viruses; wildlife; yaks; Zoology; China; Influenza Virus; Avian Influenza Virus; Hemagglutination Inhibition; Competitive ELISA; Influenza
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-015-2724-4

We conducted a serological survey to detect antibodies against avian influenza virus (AIV) in Gazella subgutturosa, Canis lupus, Capreolus pygargus, Sus scrofa, Cervus elaphus, Capra ibex, Ovis ammon, Bos grunniens and Pseudois nayaur in Xinjiang, China. Two hundred forty-six sera collected from 2009 to 2013 were assayed for antibodies against H5, H7 and H9 AIVs using hemagglutination inhibition (HI) tests and a pan-influenza competitive ELISA. Across all tested wildlife species, 4.47 % harbored anti-AIV antibodies that were detected by the HI assay. The seroprevalence for each AIV subtype across all species evaluated was 0 % for H5 AIV, 0.81 % for H7 AIV, and 3.66 % for H9 AIV. H7-reactive antibodies were found in Canis lupus (9.09 %) and Ovis ammon (4.55 %). H9-reactive antibodies were found in Gazella subgutturosa (4.55 %), Canis lupus (27.27 %), Pseudois nayaur (23.08 %), and Ovis ammon (4.55 %). The pan-influenza competitive ELISA results closely corresponded to the cumulative prevalence of AIV exposure as measured by subtype-specific HI assays, suggesting that H7 and H9 AIV subtypes predominate in the wildlife species evaluated. These data provide evidence of prior infection with H7 and H9 AIVs in non-avian wildlife in Xinjiang, China.