Effect of the application of a casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) paste and adhesive systems on bond durability of a fissure sealant

• Published in: Odontology Vol. 101; no. 1; pp. 52 - 59
• Main Authors: Borges, Boniek Castillo Dutra, Catelan, Anderson, Sasaki, Robson Tetsuo, Ambrosano, Gláucia Maria Bovi, Reis, André Figueiredo, Aguiar, Flávio Henrique Baggio
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 2013; Springer Nature B.V
• Subjects: Adhesives; Analysis of Variance; Caseins - chemistry; Dental Bonding - methods; Dental Cements - chemistry; Dental Enamel - drug effects; Dental Etching - methods; Dental research; Dental Restoration Failure; Dentistry; Humans; Medicine; Molar, Third; Oral and Maxillofacial Surgery; Original Article; Pit and Fissure Sealants - chemistry; Sealing compounds; Tensile Strength; Fissure sealant; Casein phosphopeptide–amorphous calcium phosphate; Preventive dentistry; Tensile strength; Dental adhesives
• ISSN: 1618-1247; 1618-1255
• DOI: 10.1007/s10266-012-0062-5

This study aimed to evaluate the effect of the previous application of a casein phosphopeptide–amorphous calcium phosphate paste (MI Paste, MI) and adhesive systems on the bond durability of a fissure sealant. Ninety-eight enamel blocks were obtained from proximal surfaces of erupted third molars. Specimens were divided into 14 groups ( n  = 7) according to the previous application of MI (with and without) and the adhesive systems used (no adhesive system; hydrophobic resin of a three-step etch-and-rinse adhesive system; etch-and-rinse single-bottle adhesive system; all-in-one adhesive system; two-step self-etching adhesive system; additional phosphoric acid conditioning and all-in-one adhesive system; additional phosphoric acid conditioning and two-step self-etching adhesive system). A fissure sealant (Fluroshield) was applied and photoactivated for 20 s. Beams (~0.7 mm 2 ) were prepared for the microtensile bond strength test, which was executed after 24 h or 6 months of water storage. Fractured specimens were analyzed by scanning electronic microscopy. Data were analyzed by two-way ANOVA with repeated measures/Tukey’s test ( P  < 0.05). Groups that received MI application and adhesive systems presented higher means than those groups where MI was not applied. Higher frequency of cohesive failures was observed for groups with MI. Applying a CPP–ACP containing paste on enamel before adhesive systems was an effective method to increase bond durability of the sealant tested.