Proteomics of organelles and large cellular structures

The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights...

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Bibliographic Details
Published inNature reviews. Molecular cell biology Vol. 6; no. 9; pp. 702 - 714
Main Authors Yates III, John R, Gilchrist, Annalyn, Howell, Kathryn E, Bergeron, John J. M
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.09.2005
Subjects
Biology
Cell Fractionation
Chromatography, Liquid
Cytoplasm
Fractionation
Mass Spectrometry
Organelles - chemistry
Organelles - metabolism
Proteins
Proteome - analysis
Proteomics
United States > US
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Summary:The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights into the biology and functions of these structures. Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies.
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ISSN:1471-0072
1471-0080
DOI:10.1038/nrm1711