Identification and nucleotide sequence of the thymidine kinase gene of canine herpesvirus

• Published in: Virus genes Vol. 12; no. 2; p. 185
• Main Authors: Xuan, X. (University of Tokyo, Tokyo, Japan.), Maeda, K, Tohya, Y, Mikami, T, Otsuka, H
• Format: Journal Article
• Language: English
• Published: United States 1996
• Subjects: Amino Acid Sequence; AMINO ACID SEQUENCES; Animals; ARN MENSAJERO; ARN MESSAGER; Base Sequence; BINDING SITES; Cell Line; CHEMICAL COMPOSITION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CROSS HYBRIDIZATION; DNA HYBRIDIZATION; DNA, Viral; Dogs; EXPRESION GENICA; EXPRESSION DES GENES; FELINE HERPESVIRUS; GENBANK/D83054; GENE; GENE EXPRESSION; GENES; GENETICA; GENETICS; GENETIQUE; Herpesvirus 1, Canid - enzymology; HERPETOVIRIDAE; HIBRIDACION DE ADN; HYBRIDATION D'ADN; MESSENGER RNA; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; OPEN READING FRAMES; RESTRICTION MAPPING; RNA, Viral - analysis; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; STRUCTURAL GENES; THYMIDINE; Thymidine Kinase - genetics; TIMIDINA; TRANSFERASAS; TRANSFERASE; TRANSFERASES
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/bf00572957

This paper presents the entire nucleotide sequence of the thymidine Kinase (TK) gene of canine herpesvirus (CHV). The gene was located within a 2.1 kbp EcoRV fragment by Southern-blot hybridization with a probe derived from the known feline herpesvirus type 1 (FHV-1) TK gene. An open reading frame (ORF) of 987 nucleotides, capable of encoding a TK translation product of 328 amino acids, was identified. Alignment of the predicted amino acid sequence of the CHV TK with other herpesvirus TKs revealed homologies of 25-47%. The proposed nucleotide-binding site and thymidine-binding site sequences of known herpesvirus TKs could be aligned with similar sequences in CHV TK. Northern-blot analysis revealed 1.3 kb and 5.0 kb mRNAs as the TK-specific transcripts. It is probable that the 1.3 kb transcript codes for the CHV TK and that the 5.0 kb transcript codes for the CHV TK and the downstream sequence.