Experimental study of the biological properties of nmHA-SiO2 fiber materials prepared by electrospinning technology

• Published in: Dental Materials Journal Vol. 43; no. 4; pp. 495 - 503
• Main Authors: ZHANG, Wenyun, ZHENG, Yuhan, YANG, Cheng, YU, Zhimin, ZHAO, Yuan, YANG, Li, LI, Yanbo, LIU, Qing, XU, Chongyan, SU, Jun, YAN, Tingting
• Format: Journal Article
• Language: English
• Published: Tokyo The Japanese Society for Dental Materials and Devices 25.07.2024; Japanese Society for Dental Materials and Devices; Japan Science and Technology Agency
• Subjects: Biocompatibility; Biological properties; Bone biomaterials; Bone growth; Cell surface; Cholecystokinin; Computed tomography; Cytotoxicity; Defects; Guided bone regeneration; Implantation; Materials substitution; Membranes; Multivariate analysis; Nanohydroxyapatite; Orthopaedic implants; Rabbits; Regeneration; Regeneration (physiology); Silica; Silicon dioxide; Skull defect; Staining; Substitute bone; Variance analysis
• ISSN: 0287-4547; 1881-1361; 1881-1361
• DOI: 10.4012/dmj.2023-274

To study the biocompatibility of nanohydroxyapatite (nmHA)-SiO2 fiber material and its efficacy in guided bone regeneration. ① The cytotoxicity of the nmHA-SiO2 fiber material to MC3T3-E1 cells was determined by CCK-8 assay. The adhesion of cells on the surface of the material was observed. ② Bone defects were prepared in the skull of three groups of New Zealand white rabbits. The following treatments were administered: implantation of nmHA-SiO2, implantation of Bio-Oss, and no treatment. The defects were then covered with nmHA-SiO2 membrane or Hai’ao oral repair membrane. Animal samples were analyzed by gross observation, micro-computed tomography, hematoxylin-eosin staining and Masson staining. The data were statistically analyzed by multivariate analysis of variance to evaluate the repair of bone defects. ① The nmHA-SiO2 fiber material has suitable biocompatibility. ② The nmHA-SiO2 fiber material performed more effectively as a barrier membrane than other bone substitute materials in GBR model rabbits.