In vitro uptake and release of natamycin Dex-b-PLA nanoparticles from model contact lens materials

• Published in: Journal of biomaterials science. Polymer ed. Vol. 25; no. 1; pp. 18 - 31
• Main Authors: Phan, Chau-Minh, Subbaraman, Lakshman, Liu, Shengyan, Gu, Frank, Jones, Lyndon
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.01.2014
• Subjects: antifungal; Antifungal Agents - chemistry; Biomedical materials; Contact; contact lens; Contact Lenses; Dextrans - chemistry; Drug Carriers - chemistry; drug delivery; Drugs; Encapsulation; Hydrogels - chemistry; Lenses; nanoparticle; Nanoparticles; Nanoparticles - chemistry; natamycin; Natamycin - chemistry; PHEMA; Polyesters - chemistry; silicone hydrogel; Silicones - chemistry; Uptakes
• ISSN: 0920-5063; 1568-5624
• DOI: 10.1080/09205063.2013.830914

Purpose: To evaluate the uptake and release of the antifungal agent natamycin encapsulated within poly(D,L-lactide)-dextran nanoparticles (Dex-b-PLA NPs) from model contact lens (CL) materials. Methods: Six model CL materials (gel 1:poly(hydroxyethyl methacrylate, pHEMA); gel 2:85% pHEMA: 15% [Tris(trimethylsiloxy)silyl]-propyl methacrylate (TRIS); gel 3: 75% pHEMA: 25% TRIS; gel 4: 85% N,N dimethylacrylamide (DMAA): 15% TRIS; gel 5:75% DMAA: 25% TRIS; and gel 6: DMAA) were prepared using a photoinitiation procedure. The gels were incubated in: (1) natamycin dissolved in deionized (DI) water and (2) natamycin encapsulated within Dex-b-PLA NPs in dimethylsulfoxide/DI water. Natamycin release from these materials was monitored using UV-visible spectrophotometry at 304 nm over 7 d. Results: Natamycin uptake by all model CL materials increased between 1 and 7 d (p < 0.001). The uptake of natamycin-NPs was higher than the uptake of the drug alone in DI water (p < 0.05). Drug release was higher in materials containing DMAA than pHEMA (p < 0.05). All gels loaded with natamycin-NPs also released more drug compared to gels soaked with natamycin in DI water (p < 0.001). After 1 h, CL materials loaded with natamycin alone released 28-82% of the total drug release. With the exception of gel 6, this burst released was reduced to 21-54% for CL materials loaded with natamycin-NPs. Conclusions: Model CL materials loaded with natamycin-Dex-b-PLA NPs were able to release natamycin for up to 12 h under infinite sink conditions. DMAA-TRIS materials may be more suitable for drug delivery of natamycin due to the higher drug release observed with these materials.