Role of Tissue-Specific Blood Flow and Tissue Recruitment in Insulin-Mediated Glucose Uptake of Human Skeletal Muscle

• Published in: Circulation (New York, N.Y.) Vol. 98; no. 3; pp. 234 - 241
• Main Authors: Bonadonna, Riccardo C., Saccomani, Maria Pia, Del Prato, Stefano, Bonora, Enzo, DeFronzo, Ralph A., Cobelli, Claudio
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 21.07.1998; American Heart Association, Inc
• Subjects: Adult; Biological and medical sciences; Blood Glucose - analysis; Cardiovascular system; Extracellular Space - metabolism; Female; Forearm - blood supply; Glucose - metabolism; Glucose Clamp Technique; Humans; Insulin - blood; Insulin - pharmacology; Investigative techniques of hemodynamics; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Muscle, Skeletal - metabolism; Osmolar Concentration; Regional Blood Flow - drug effects; Human; Exploration; Glucose; Metabolism; Striated muscle; Insulin; Capture; Blood flow; Regulation(control); Blood vessel; Circulatory system; Hemodynamics; Radial artery
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.98.3.234

Background —Conflicting evidence exists concerning whether insulin-induced vasodilation plays a mechanistic role in the regulation of limb glucose uptake. It can be predicted that if insulin augments blood flow by causing tissue recruitment, this mechanism would enhance limb glucose uptake. Methods and Results —Twenty healthy subjects were studied with the forearm perfusion technique in combination with the euglycemic insulin clamp technique. Ten subjects were studied at physiological insulin concentrations (≈400 pmol/L) and the other 10 at supraphysiological insulin concentrations (≈5600 pmol/L). Four additional subjects underwent a saline control study. Pulse injections of a nonmetabolizable extracellular marker (1-[ 3 H]- l -glucose) were administered into the brachial artery, and its washout curves were measured in one ipsilateral deep forearm vein and used to estimate the extracellular volume of distribution and hence the amount of muscle tissue drained by the deep forearm vein. Both during saline infusion and at physiological levels of hyperinsulinemia we observed no changes in blood flow and/or muscle tissue drained by the deep forearm vein. However, supraphysiological hyperinsulinemia accelerated total forearm blood flow (45.0±1.8 versus 36.5±1.3 mL · min −1 · kg −1 , P <0.01) and increased the amount of muscle tissue drained by the deep forearm vein (305±46 versus 229±32 g, P <0.05). The amount of tissue newly recruited by insulin was strongly correlated to the concomitant increase in tissue glucose uptake ( r =0.789, P <0.01). Conclusions —Acceleration of forearm blood flow mediated by supraphysiological hyperinsulinemia is accompanied by tissue recruitment, which may be a relevant determinant of forearm (muscle) glucose uptake.