Testosterone metabolism in Neomysis integer following exposure to benzo( a)pyrene

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 144; no. 4; pp. 405 - 412
• Main Authors: Poelmans, S., Verslycke, T., Monteyne, E., Noppe, H., Verheyden, K., Janssen, C.R., De Brabander, H.F.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.08.2006
• Subjects: Animals; Benzo( a)pyrene; Benzo(a)pyrene - toxicity; Biomarker; Chromatography, Liquid; Crustacea; Crustacea - drug effects; Crustacea - metabolism; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450 modulator; Endocrine Disruptors - toxicity; Eubacteria; Invertebrates; Mass Spectrometry; Mysidacea; Neomysis integer; Testosterone - metabolism; Testosterone metabolism; Biomarker; Cytochrome P450 modulator; Benzo( a)pyrene; Invertebrates; Neomysis integer
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2006.04.001

Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function. Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this study was to investigate the potential effects of the known CYP modulator, benzo( a)pyrene (B( a)P), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96 h to 0.43, 2.39, 28.83, 339.00 and 1682.86 μg B( a)P L − 1 and a solvent control, and subsequently their ability to metabolize testosterone was assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC–MS 2). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1682.86 μg B( a)P L − 1 as compared to the control animals.