Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic ( Allium sativum L.)

• Published in: Scientia horticulturae Vol. 122; no. 3; pp. 355 - 361
• Main Authors: Ma, Kyung-Ho, Kwag, Jae-Gyun, Zhao, Weiguo, Dixit, Anupam, Lee, Gi-An, Kim, Haeng-Hoon, Chung, Ill-Min, Kim, Nam-Soo, Lee, Jae-Sun, Ji, Jae-Jun, Kim, Tae-San, Park, Yong-Jin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2009; [Amsterdam; New York, NY]: Elsevier Science; Elsevier
• Subjects: Agronomy. Soil science and plant productions; alleles; Allium sativum; Allium sativum L; Biological and medical sciences; cultivar identification; DNA fingerprinting; Fundamental and applied biological sciences. Psychology; Garlic; Generalities. Genetics. Plant material; Genetic diversity; genetic markers; genetic relationships; Genetic resources, diversity; genetic variation; Genetics and breeding of economic plants; genome; germplasm screening; heterozygosity; loci; microsatellite repeats; molecular sequence data; Plant material; population genetics; SSRs; Garlic; Genetic diversity; Allium sativum L; SSRs; Monocotyledones; Horticulture; Medicinal plant; Liliaceae; Microsatellite DNA; Angiospermae; Flavoring crop; Spermatophyta; Genome; Allium sativum; Polymorphism
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2009.06.010

Here we characterized eight novel polymorphic SSR markers, developed from an enriched genomic library of garlic ( Allium sativum L.). These SSRs produced a total of 64 alleles across 90 garlic accessions, with an average of 8 alleles per locus. Values for observed ( H O) and expected ( H E) heterozygosity ranged from 0.16 to 0.77 (mean = 0.44) and from 0.22 to 0.86 (mean = 0.65), respectively. Six loci deviated significantly ( P < 0.05) from Hardy–Weinberg equilibrium (HWE). The averages of gene diversity and PIC values were 0.65 and 0.62, respectively. The mean genetic similarity coefficient was 0.4380, indicating that among garlic accessions existed wide genetic variation. Based on 64 alleles obtained by 8 SSRs, a phenogram was constructed to understand the relationships among the 90 accessions. These newly developed SSRs should prove very useful tools for genotypes identification, assessment of genetic diversity and population structure in garlic.