Mec -Positive Staphylococcus Healthcare-Associated Infections Presenting High Transmission Risks for Antimicrobial-Resistant Strains in an Equine Hospital

• Published in: Antibiotics (Basel) Vol. 11; no. 5; p. 621
• Main Authors: Soza-Ossandón, Paula, Rivera, Dácil, Allel, Kasim, González-Rocha, Gerardo, Quezada-Aguiluz, Mario, San Martin, Ivan, García, Patricia, Moreno-Switt, Andrea I
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.05.2022; MDPI
• Subjects: Animal health; Animals; Antibiotic resistance; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Coagulase; Drug resistance; Electrophoresis; Epidemiology; Evaluation; Fomites; Gel electrophoresis; Health care; healthcare-associated infections; Hospitalization; Hospitals; Infections; MecA protein; Medical equipment; Methicillin; methicillin resistance; Multidrug resistance; Multidrug resistant organisms; one health; Oxacillin; Pathogens; Patients; Public health; Regression analysis; Risk; Staphylococcus; Staphylococcus infections; Veterinary medicine; Winter; methicillin resistance; one health; Staphylococcus; antimicrobial-resistance; healthcare-associated infections
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics11050621

Healthcare-associated infections caused by , particularly , represent a high risk for human and animal health. can be easily transmitted through direct contact with individual carriers or fomites, such as medical and non-medical equipment. The risk increases if strains carry antibiotic resistance genes and show a phenotypic multidrug resistance behavior. The aim of the study was to identify and characterize methicillin resistant coagulase-positive staphylococci (MRSA) and coagulase-negative staphylococci (MRCoNS) in equine patients and environmental sources in an equine hospital to evaluate the genetic presence of multidrug resistance and to understand the dissemination risks within the hospital setting. We explored 978 samples for MRSA and MRCoNS using Oxacillin Screen Agar in an equine hospital for racehorses in Chile, which included monthly samples ( = 61-70) from equine patients (246) and hospital environments (732) in a one-year period. All isolates were PCR-assessed for the presence of methicillin resistance gene and/or . Additionally, we explored the epidemiological relatedness by Pulsed Field Gel Electrophoresis (PFGE) in MRSA isolates. Phenotypic antibiotic resistance was evaluated using the Kirby-Bauer disk diffusion method. We estimated the unadjusted and adjusted risk of acquiring drug-resistant strains by employing logistic regression analyses. We identified 16 MRSA isolates and 36 MRCoNS isolates. For MRSA, we detected A and C in 100% and 87.5 % of the isolates, respectively. For MRCoNS, A was detected among 94% of the isolates and among 86%. MRSA and MRCoNS were isolated from eight and 13 equine patients, respectively, either from colonized areas or compromised wounds. MRSA strains showed six different pulse types (i.e., A1-A3, B1-B2, C) isolated from different highly transited areas of the hospital, suggesting potential transmission risks for other patients and hospital staff. The risk of acquiring drug-resistant species is considerably greater for patients from the surgery, equipment, and exterior areas posing higher transmission risks. Tackling antimicrobial resistance (AMR) using a One Health perspective should be advocated, including a wider control over antimicrobial consumption and reducing the exposure to AMR reservoirs in animals, to avoid cross-transmission of AMR within equine hospitals.