Applications of Raman spectroscopy in the development of cell therapies: state of the art and future perspectives
Therapies based on injecting living cells into patients offer a huge potential to cure many degenerative and deadly diseases, with hundreds of clinical trials ongoing. Due to their complex nature, a basic understanding of their biochemical and functional characteristics, how to manufacture them for...
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| Published in | Analyst (London) Vol. 145; no. 6; pp. 27 - 215 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Royal Society of Chemistry
16.03.2020
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Therapies based on injecting living cells into patients offer a huge potential to cure many degenerative and deadly diseases, with hundreds of clinical trials ongoing. Due to their complex nature, a basic understanding of their biochemical and functional characteristics, how to manufacture them for safe and efficacious therapy, and how to effectively implement them in clinical settings are very challenging. Raman spectroscopy could provide an information-rich, non-invasive, non-destructive analytical method to complement the use of conventional sample-based, infrequent and destructive biochemical assays typically employed to analyze and validate the quality of therapeutic cells. This article provides an overview of the current state of emerging cell therapies, and then reviews the related Raman spectroscopic state of the art analysis of human cells. This includes spectroscopic data processing considerations, the scope offered by technical variants of Raman spectroscopy, and analytical difficulties encountered by spectroscopists working with therapeutic cells. Finally, we outline a number of salient challenges as cell therapy products are translated from the laboratory to the clinic, and propose how Raman spectroscopy-based solutions could address these challenges.
This comprehensive review article discusses current and future perspectives of Raman spectroscopy-based analyses of cell therapy processes and products. |
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| Bibliography: | Shreyas Rangan completed his B.Tech. and M.Tech. in Biotechnology from the Indian Institute of Technology Madras, followed by an M.Sc. in Genome Science and Technology from The University of British Columbia (UBC). He is currently a Ph.D. Candidate at UBC's School of Biomedical Engineering, completing his thesis research focused on Raman spectroscopic analyses of cells for therapy in the laboratories of Prof. James Piret and Prof. Robin Turner. Georg Schulze obtained a Bsc. Eng. (Chem.) from the University of Pretoria, followed by two years of industrial experience. He then returned for a M.A. (Psychology) and a Ph.D. (Interdisciplinary Studies) at The University of British Columbia where he focused on bioanalytical applications of Raman spectroscopy and related spectral processing techniques. He continued this work as a Research Associate at the Michael Smith Laboratories at The University of British Columbia until his recent retirement. Michael Blades received a BSc in Chemistry at St. Mary's University (Halifax, Nova Scotia) and a PhD at the University of Alberta in 1980 working in the area of plasma spectrochemistry. He joined the Department of Chemistry at The University of British Columbia in Vancouver 1981 and retired in 2018. His current research interests are in the area Raman microspectroscopy for biophysical and bioanalytical measurements. He has also served as Editor (2009-2012, 2018-present) and Editor-in-Chief (2012-2018) for Martha Vardaki obtained a B.Sc. and M.Sc. in Pharmacy and Pharmaceutical analysis respectively from the University of Patras, Greece. She then pursued a Ph.D. degree in physics from the University of Exeter (UK), under the supervision of Prof. N. Stone and P. Matousek, followed by a Research Associate appointment in Imperial College London. She is currently a Postdoctoral Research fellow in the Michael Smith Laboratories at The University of British Columbia. Her research focuses on biomedical applications of conventional and deep Raman spectroscopy. Robin Turner earned a Ph.D. degree in electrical engineering from the University of Alberta in 1990. He is now a Professor at The University of British Columbia with joint appointments in the Michael Smith Laboratories and Department of Electrical & Computer Engineering; he is also an Associate Member of the Department of Chemistry. His current research activities focus on applications of Raman spectroscopy to analytical problems in biochemistry, biotechnology, and biomedical engineering. James Piret has a Bachelor's degree from Harvard in Applied Mathematics to Biochemistry and a Chemical Engineering doctoral degree from MIT in 1989. He is a Professor at The University of British Columbia in the Department of Chemical & Biological Engineering and the Michael Smith Laboratories; he is also an Associate Member of the School of Biomedical Engineering. His research focus is on innovative process and device technology development for mammalian cell culture therapeutic protein or cell manufacturing. . Applied Spectroscopy ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
| ISSN: | 0003-2654 1364-5528 |
| DOI: | 10.1039/c9an01811e |