In Vitro Schistosomicidal Activity of Some Brazilian Cerrado Species and Their Isolated Compounds

• Published in: Evidence-based complementary and alternative medicine Vol. 2012; no. 2012; pp. 1 - 8
• Main Authors: Groppo, Milton, da Silva Filho, Ademar Alves, Andrade e Silva, Márcio Luís, Cunha, Wilson Roberto, Pauletti, Patrícia Mendonça, Januário, Ana Helena, Gimenez, Valéria Maria Meleiro, Magalhães, Lizandra Guidi, Silva, Claudia Peres, Peixoto, Juliana Andrade, Souza, Herbert Cristian de, Cintra, Lucas Silva, Uchôa, Camila Jacintho de Mendonça, Cunha, Nayanne Larissa, Rodrigues, Vanderlei
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2012; Hindawi Limited
• Subjects: Araliaceae; Biodiversity; Death; Flavonoids; L-Rhamnopyranoside; Loranthaceae; Melastomataceae; Miconia; Motor activity; n-Hexane; Oleanolic acid; Parasites; Parasitology; Proteaceae; Quercetin; Schefflera; Schistosoma; Schistosoma mansoni; Species; Stomphia vinosa; Triterpenes; Tropical diseases; Ursolic acid; Worms; Brazil; Cerrado Biome
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2012/173614

Miconia langsdorffii Cogn. (Melastomataceae), Roupala montana Aubl. (Proteaceae), Struthanthus syringifolius (Mart.) (Loranthaceae), and Schefflera vinosa (Cham. & Schltdl.) Frodin (Araliaceae) are plant species from the Brazilian Cerrado whose schistosomicidal potential has not yet been described. The crude extracts, fractions, the triterpenes betulin, oleanolic acid, ursolic acid and the flavonoids quercetin 3-O-β-D-rhamnoside, quercetin 3-O-β-D-glucoside, quercetin 3-O-β-D-glucopyranosyl-(1-2)-α-L-rhamnopyranoside and isorhamnetin 3-O-β-D-glucopyranosyl-(1-2)-α-L-rhamnopyranoside were evaluated in vitro against Schistosoma mansoni adult worms and the bioactive n-hexane fractions of the mentioned species were also analyzed by GC-MS. Betulin was able to cause worm death percentage values of 25% after 120 h (at 100 μM), and 25% and 50% after 24 and 120 h (at 200 μM), respectively; besides the flavonoid quercetin 3-O-β-D-rhamnoside promoted 25% of death of the parasites at 100 μM. Farther the flavonoids quercetin 3-O-β-D-glucoside and quercetin 3-O-β-D-rhamnoside at 100 μM exhibited significantly reduction in motor activity, 75% and 87.5%, respectively. Biological results indicated that crude extracts of R. montana, S. vinosa, and M. langsdorffii and some n-hexane and EtOAc fractions of this species were able to induce worm death to some extent. The results suggest that lupane-type triterpenes and flavonoid monoglycosides should be considered for further antiparasites studies.