Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes

• Published in: Sensors and actuators. B, Chemical Vol. 214; pp. 50 - 55
• Main Authors: Guo, Yahui, Wang, Qinglin, Wang, Zhili, Chen, Xing, Xu, Lijun, Hu, Jiming, Pei, Renjun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2015
• Subjects: Actuators; Deoxyribonucleic acid; Displacement; DNA ligase; Genetics; Kinases; Label free; Monitoring; Polynucleotide kinase; Split G-quadruplex; Strands; Strategy; Toehold-mediated strand displacement; DNA ligase; Label free; Polynucleotide kinase; Split G-quadruplex; Toehold-mediated strand displacement

Herein, an efficient pair of split G-quadruplex probes was selected (S/N=21) to construct a simple, rapid and label-free technique for the detections of T4 DNA ligase and PNK activity. DNA ligase and PNK are engaged in DNA replication and repair, ensuring the genetic stability. The detections of T4...