Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes

Herein, an efficient pair of split G-quadruplex probes was selected (S/N=21) to construct a simple, rapid and label-free technique for the detections of T4 DNA ligase and PNK activity. DNA ligase and PNK are engaged in DNA replication and repair, ensuring the genetic stability. The detections of T4...

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Published inSensors and actuators. B, Chemical Vol. 214; pp. 50 - 55
Main Authors Guo, Yahui, Wang, Qinglin, Wang, Zhili, Chen, Xing, Xu, Lijun, Hu, Jiming, Pei, Renjun
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.07.2015
Subjects
Actuators
Deoxyribonucleic acid
Displacement
DNA ligase
Genetics
Kinases
Label free
Monitoring
Polynucleotide kinase
Split G-quadruplex
Strands
Strategy
Toehold-mediated strand displacement
DNA ligase
Label free
Polynucleotide kinase
Split G-quadruplex
Toehold-mediated strand displacement
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Summary:Herein, an efficient pair of split G-quadruplex probes was selected (S/N=21) to construct a simple, rapid and label-free technique for the detections of T4 DNA ligase and PNK activity. DNA ligase and PNK are engaged in DNA replication and repair, ensuring the genetic stability. The detections of T4 ligase and PNK activity are of great significance in biology and biomedicine. Split G-quadruplex probes are robust label-free biosensing tools with turn-on signal readout. The selected pair of split G-quadruplex probes combined with toehold-mediated strand displacement in this work demonstrated an efficient strategy for monitoring the activities of T4 DNA ligase and PNK with good sensitivity.
Bibliography:ObjectType-Article-1
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ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2015.03.013