Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells

The regeneration of articular cartilage posed a formidable challenge due to the restricted treatment efficacy of existing therapies. Scaffold-based tissue engineering emerges as a promising avenue for cartilage reconstitution. However, most scaffolds exhibit inadequate mechanical characteristics, po...

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Published inCollagen and Leather Vol. 6; no. 1; pp. 3 - 12
Main Authors Zhang, Xinyue, Zhan, Xue, Cheng, Haojin, Dong, Zuqin, Hu, Chen, Liu, Chenxin, Liang, Jie, Chen, Yafang, Fan, Yujiang, Zhang, Xingdong
Format Journal Article
LanguageEnglish
Published Singapore Springer Nature Singapore 01.12.2024
Springer Nature B.V
SpringerOpen
Subjects
Articular cartilage
Biocompatibility
Bone marrow
Cartilage
Cell adhesion
Chemistry and Materials Science
Chondrogenic differentiation
Collagen
Crosslinking
Differentiation
Dopamine hydrochloride
Glycosaminoglycans
Hyaluronic acid
Hydrogels
Injectability
Materials Engineering
Materials Science
Mechanical properties
Polysaccharides
Proteins
Recombinant humanized collagen
Scaffolds
Stem cells
Tissue engineering
Dopamine hydrochloride
Chondrogenic differentiation
Recombinant humanized collagen
Articular cartilage
Hyaluronic acid
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Summary:The regeneration of articular cartilage posed a formidable challenge due to the restricted treatment efficacy of existing therapies. Scaffold-based tissue engineering emerges as a promising avenue for cartilage reconstitution. However, most scaffolds exhibit inadequate mechanical characteristics, poor biocompatibility, or absent cell adhesion sites. In this study, cartilage-like protein-polysaccharide hybrid hydrogel based on DOPA-modified hyaluronic acid, bovine type I collagen (Col I), and recombinant humanized type II collagen (rhCol II), denoted as HDCR. HDCR hydrogels possessed the advantage of injectability and in situ crosslinking through pH adjustment. Moreover, HDCR hydrogels exhibited a manipulable degradation rate and favorable biocompatibility. Notably, HDCR hydrogels significantly induced chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells in vitro, as demonstrated by the upregulation of crucial chondrogenic genes (type II collagen, aggrecan) and the abundant accumulation of glycosaminoglycan. This approach presented a strategy to manufacture injectable, biodegradable scaffolds based on cartilage-like protein-polysaccharide polymers, offering a minimally invasive solution for cartilage repair. Graphical abstract
ISSN:2097-1419
2731-6998
2524-7859
DOI:10.1186/s42825-023-00146-2