The ubiquitin-proteasome pathway regulates claudin 5 degradation
The tight junctions (TJs) form continuous intracellular contacts, which help create selective barriers in epithelial and endothelial cell layers. The structures created by the TJs are very dynamic and can be rapidly remodeled in response to physiological and pathological signals. Claudin 5 is a memb...
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Published in | Journal of cellular biochemistry Vol. 113; no. 7; pp. 2415 - 2423 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.07.2012
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Subjects | |
Online Access | Get full text |
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Summary: | The tight junctions (TJs) form continuous intracellular contacts, which help create selective barriers in epithelial and endothelial cell layers. The structures created by the TJs are very dynamic and can be rapidly remodeled in response to physiological and pathological signals. Claudin 5 is a membranal TJ protein which plays a critical role in determining the permeability of endothelial barriers. We describe the regulation of claudin 5 degradation by the ubiquitin–proteasome system (UPS). Our results indicate that claudin 5 has a relatively short half‐life and can be polyubiquitinated on lysine 199. This ubiquitination appears to trigger the proteasome‐dependent degradation of claudin 5. Other mechanisms also seem to be involved in the post‐translational regulation of claudin 5, including a ubiquitin‐independent and probably indirect lysosomal‐dependent pathway. These findings provide evidence for the involvement of the UPS in the regulation of claudin 5 levels, and set the stage for further research to determine the involvement of this pathway in the modulation of the properties of TJs and cell‐layer barriers. J. Cell. Biochem. 113: 2415–2423, 2012. © 2012 Wiley Periodicals, Inc. |
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Bibliography: | ark:/67375/WNG-GBZMGTNP-7 Rappaport Faculty of Medicine and Research Institute, Technion-Israel Institute of Technology ArticleID:JCB24118 Work presented in this manuscript was performed at the Rappaport Faculty of Medicine and Research Institute, Technion-Israel Institute of Technology, and Division of Neuroimmunology, Carmel Medical Center, Haifa, Israel. istex:946D1B48651FDAEF48FE634DAB88357E92B6D67C Work presented in this manuscript was performed at the Rappaport Faculty of Medicine and Research Institute, Technion‐Israel Institute of Technology, and Division of Neuroimmunology, Carmel Medical Center, Haifa, Israel. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0730-2312 1097-4644 |
DOI: | 10.1002/jcb.24118 |