Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation

With the renewed enthusiasm in immuno-oncology, characterization of the tumor immune microenvironment constitutes an essential and unique aspect to the assessment of therapeutics. The isolation of tumor-infiltrating lymphocytes (TILs) is a desirable approach toward the understanding of antitumor imm...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1960; p. 93
Main Authors Tan, Yee Sun, Lei, Yu L
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Mechanical dissociation
Tumor microenvironment
Tumor-infiltrating lymphocytes
Immune escape
Immunotherapy
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Summary:With the renewed enthusiasm in immuno-oncology, characterization of the tumor immune microenvironment constitutes an essential and unique aspect to the assessment of therapeutics. The isolation of tumor-infiltrating lymphocytes (TILs) is a desirable approach toward the understanding of antitumor immune response. This chapter provides an effective protocol to mechanically dissociate tumor tissue and generate single-cell suspension from excised tumors. TILs are then isolated by Ficoll-Paque density gradient centrifugation. This protocol is applicable to both human and experimental tumors in immunocompetent murine models.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9167-9_8