Immunocytochemical evaluation of thyroid neoplasms on thin‐layer smears from fine‐needle aspiration biopsies
BACKGROUND Fine‐needle aspiration biopsy (FNAB) is the most reliable diagnostic tool for thyroid nodules. A difficult cytologic diagnosis may be supported by an immunocytochemical study. The efficacy of a panel made up of RET, HBME‐1, and Galectin‐3 antibodies was evaluated in smears processed by th...
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Published in | Cancer Vol. 105; no. 2; pp. 87 - 95 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
25.04.2005
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Subjects | |
Online Access | Get full text |
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Summary: | BACKGROUND
Fine‐needle aspiration biopsy (FNAB) is the most reliable diagnostic tool for thyroid nodules. A difficult cytologic diagnosis may be supported by an immunocytochemical study. The efficacy of a panel made up of RET, HBME‐1, and Galectin‐3 antibodies was evaluated in smears processed by thin‐layer cytology (TLC).
DESIGN
Thyroid FNABs (n = 99) with both conventional (CS) and thin‐layer cytology (TLC) smears were studied. The cases were diagnosed as follows: 5 benign lesions (BL), 13 papillary carcinomas (PC), and 81 follicular proliferations (FP). The category of FP was divided into three subgroups according to nuclear features of follicular cells: Follicular neoplasm (FN NOS), oxyphilic follicular neoplasm (OFN), and folliucular lesion with nuclear pleomorphism (FLWNP). Immunostains for HBME‐1, Galectin‐3, and RET were carried out on TLC slides.
RESULTS
Among 49 cases undergoing surgery, all 10 PC and 2 BL were histologically confirmed, whereas 15 out of 37 FP (40.5%) were malignant. The complete immunocytochemical panel (ICCP) was positive in 9 of 10 malignancies (90%) and negative in both BLs. Out of 37 FP, the ICCP yielded positive in 15 cases (4 benign, 11 malignant) and negative in 11 (all benign). In the FLWNP subgroup, the ICCP was positive in 11 (84.6%) and negative in 2 histologically benign cases.
CONCLUSION
The combined panel of antibodies and the nuclear pleomorphism of follicular cells were effective in distinguishing between thyroid nodules requiring surgery from thyroid nodules requiring just follow‐up. Cancer (Cancer Cytopathol) 2005. © 2005 American Cancer Society.
A combined panel of antibodies together with nuclear features of thyrocytes were effectively used to diagnose thyroid nodules that had been judged to be candidates for surgery by preoperative fine‐needle biopsy. Immunocytochemistry may be performed on thin‐layer cytology with the same accuracy as that provided by conventional smears. |
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Bibliography: | Fax: (011) 39‐3015‐5883 ThinPrep® 2000 is a registered trademark of the Cytyc Corporation, Boxborough, Massachusetts, and, in this article, is the technology used for thin‐layer cytology. The Authors have no significant relationship with the manufacturer of the system used in this study. nd USCAP Meeting held in Washington, DC, March 22–28, 2003. This study was accepted as a poster for the Stowell–Orbison Award during the 92 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0008-543X 1097-0142 |
DOI: | 10.1002/cncr.21026 |