Substantial Depletion of Vicine, Levodopa, and Tyramine in a Fava Bean Protein-Based Nutritional Product

• Published in: International journal of food science Vol. 2021; pp. 6669544 - 10
• Main Authors: Johns, Paul W., Hertzler, Steven R.
• Format: Journal Article
• Language: English
• Published: United States Hindawi 2021; Hindawi Limited; Wiley
• Subjects: Beans; Broad beans; Calibration; Depletion; Dopamine; High-performance liquid chromatography; Legumes; Levodopa; Liquid chromatography; Proteins; Tyramine; St Louis Missouri; United States > US
• ISSN: 2356-7015; 2314-5765; 2314-5765
• DOI: 10.1155/2021/6669544

A commercial fava bean protein isolate and a liquid nutritional product formulated with it were tested by validated HPLC methods for the favism-associated pyrimidine glycoside vicine, the dopamine precursor levodopa, and the biogenic amine tyramine. The vicine, levodopa, and tyramine concentrations in the protein isolate—306, 13.3, and <0.5 mg/kg, respectively—when expressed on a protein basis—34, 1.5, and <0.06 mg/100 g protein, respectively—were at least 96% lower than the vicine, levodopa, and tyramine (protein-based) concentrations reported for fava beans (≥900, ~200, and ~4 mg/100 g protein, respectively). This was also true for the vicine (13 mg/kg or 22 mg/100 g protein), levodopa (≤0.17 mg/kg or ≤0.3 mg/100 g protein), and tyramine (0.08 mg/kg or 0.14 mg/100 g protein) concentrations in the nutritional product. On the basis of these data, one serving (11 fl. oz.) of the nutritional product would deliver approximately 5 mg of vicine, <1 mg of levodopa, and <0.1 mg of tyramine.