Cerebrospinal fluid proteins studied by two-dimensional gel electrophoresis and immunoblotting technique

The proteins of lumbar CSF have been investigated by two-dimensional gel electrophoresis, and their patterns have been compared with the corresponding serum protein patterns. Serum proteins in CSF have been identified by electroblotting and immunoreaction with antiserum against total human serum pro...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 49; no. 2; p. 363
Main Authors Wiederkehr, F, Ogilvie, A, Vonderschmitt, D J
Format Journal Article
LanguageEnglish
Published England 01.08.1987
Subjects
Blood Proteins - analysis
Cerebrospinal Fluid Proteins - analysis
Chromatography, Gel - methods
Electrophoresis, Polyacrylamide Gel - methods
Humans
Immune Sera
Immunoassay - methods
Molecular Weight
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Summary:The proteins of lumbar CSF have been investigated by two-dimensional gel electrophoresis, and their patterns have been compared with the corresponding serum protein patterns. Serum proteins in CSF have been identified by electroblotting and immunoreaction with antiserum against total human serum proteins. Proteins derived from brain have been identified with antiserum against human brain proteins. The most prominent CSF protein group has been identified as a multiple form of apolipoprotein E. The correct position of the glial fibrillary acidic protein has also been determined. The prefractionation of CSF proteins by size exclusion chromatography or by affinity chromatography followed by two-dimensional electrophoresis has facilitated the detection of trace components in CSF and the corresponding serum.
ISSN:0022-3042
DOI:10.1111/j.1471-4159.1987.tb02874.x