Evaluation of microbiome in primary and permanent dentition in grade C periodontitis in young individuals

• Published in: Journal of periodontology (1970) Vol. 95; no. 7; p. 650
• Main Authors: Koo, Sungeun Stephanie, Fernandes, Jussara G, Li, Lu, Huang, Hong, Aukhil, Ikramuddin, Harrison, Peter, Diaz, Patricia I, Shaddox, Luciana M
• Format: Journal Article
• Language: English
• Published: United States 01.07.2024
• Subjects: Adolescent; Aggregatibacter actinomycetemcomitans - isolation & purification; Biofilms; Capnocytophaga - isolation & purification; Case-Control Studies; Child; Child, Preschool; Dentition, Permanent; DNA, Bacterial - analysis; Female; Gingiva - microbiology; Humans; Leptotrichia; Male; Microbiota; Periodontitis - microbiology; Tooth, Deciduous - microbiology; aggressive periodontitis; primary dentition; children; microbiome
• ISSN: 1943-3670
• DOI: 10.1002/JPER.23-0504

The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions. DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated. C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01). Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.